Inhibitory action of sphingosine, sphinganine and dexamethasone on glucose uptake: Studies with hydrogen peroxide and phorbol ester
- Univ. of Utah School of Medicine, Salt Lake City (USA)
The mechanism of the inhibitory action of glucocorticoids on glucose uptake is incompletely understood. Treatment with corticosteriods of cells in which glucose uptake is stimulated at insulin postbinding and postreceptor sites may clarify the site of the steroid inhibitory action. Hydrogen peroxide, which has been shown to stimulate the insulin receptor tyrosine kinase, and phorbol myristate acetate (PMA) which stimulates protein kinase C were, therefore, used as stimulators of glucose transport in this study. These studies demonstrate that dexamethasone and the sphingoid bases, sphinganine and sphingosine, inhibit glucose uptake that has been stimulated at either the receptor kinase or protein kinase C level in both 3T3-L1 and 3T3-C2 cells. These data confirm glucocorticoid inhibitory action at a post binding level and support the suggestion that some corticosteriod inhibitory effects may be mediated by an action on sphingolipid metabolism.
- OSTI ID:
- 6695426
- Journal Information:
- Life Sciences; (USA), Vol. 46:25; ISSN 0024-3205
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
GLUCOCORTICOIDS
BIOCHEMICAL REACTION KINETICS
GLUCOSE
UPTAKE
HYDROGEN PEROXIDE
BIOLOGICAL EFFECTS
PHORBOL ESTERS
DEXAMETHASONE
INHIBITION
INSULIN
PHOSPHOTRANSFERASES
RECEPTORS
ADRENAL HORMONES
ALDEHYDES
CARBOHYDRATES
CARCINOGENS
CORTICOSTEROIDS
ENZYMES
ESTERS
HEXOSES
HORMONES
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
KETONES
KINETICS
MEMBRANE PROTEINS
MONOSACCHARIDES
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
PEPTIDE HORMONES
PEROXIDES
PHOSPHORUS-GROUP TRANSFERASES
PREGNANES
PROTEINS
REACTION KINETICS
SACCHARIDES
STEROIDS
TRANSFERASES
560300* - Chemicals Metabolism & Toxicology