Inositol uptake in rat aorta

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90387-7· OSTI ID:6694977

Rapoport, R M; Van Gorp, C; Chang, Ki-Churl ^[1]

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1. Univ. of Cincinnati College of Medicine, OH (USA)

{sup 3}H-inositol uptake into deendothelialized aorta was linear for at least 2 h and was composed of both a saturable, Na{sup +}-dependent, and a nonsaturable, Na{sup +}-independent component. The Na{sup +}-dependent component of inositol uptake had a K{sub m} of 50 {mu}M and a V{sub max} of 289 pmol/mg prot/h. Exposure to LiCl, ouabain, or Ca{sup 2+} - free Krebs-Ringer bicarbonate solution inhibited uptake. Metabolic poisoning with dinitrophenol, as well as incubation with phloretin, an inhibitor of carrier-mediated hexose transport, also inhibited uptake. Exposure to norepinephrine decreased inositol uptake, while phorbol myristate acetate was without effect. Isobutylmethylxanthine significantly increased inositol uptake, while the increased uptake due to dibutyryl cyclic AMP and forskolin were not statistically significant. Sodium nitroprusside, and activator of guanylate cyclase, and 8-bromo cyclic GMP, were without effect on uptake, as was methylene blue, an inhibitor of guanylate cyclase. Inositol uptake into the aorta was increased when the endothelium was allowed to remain intact, although this effect was likely due to uptake in both the endothelial and smooth muscle cells.

• OSTI ID: 6694977
• Journal Information: Life Sciences; (USA), Vol. 46:23; ISSN 0024-3205
• Country of Publication: United States
• Language: English

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63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
59 BASIC BIOLOGICAL SCIENCES
INOSITOL
UPTAKE
NITROPHENOL
BIOLOGICAL EFFECTS
PHORBOL ESTERS
AORTA
ENDOTHELIUM
ENZYME INHIBITORS
MUSCLES
NORADRENALINE
RATS
SODIUM COMPOUNDS
TRITIUM COMPOUNDS
ADRENAL HORMONES
ALKALI METAL COMPOUNDS
ANIMAL TISSUES
ANIMALS
AROMATICS
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AUTONOMIC NERVOUS SYSTEM AGENTS
BLOOD VESSELS
BODY
CARBOHYDRATES
CARCINOGENS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
DRUGS
ESTERS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INOSITOLS
MAMMALS
MONOSACCHARIDES
NEUROREGULATORS
NITRO COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHENOLS
RODENTS
SACCHARIDES
SYMPATHOMIMETICS
TISSUES
VERTEBRATES
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