Inositol uptake in rat aorta

Journal Article · Sun Dec 31 23:00:00 EST 1989 · Life Sciences; (USA)

DOI:https://doi.org/10.1016/0024-3205(90)90387-7· OSTI ID:6694977

Rapoport, R M; Van Gorp, C; Chang, Ki-Churl ^[1]

---

1. Univ. of Cincinnati College of Medicine, OH (USA)

{sup 3}H-inositol uptake into deendothelialized aorta was linear for at least 2 h and was composed of both a saturable, Na{sup +}-dependent, and a nonsaturable, Na{sup +}-independent component. The Na{sup +}-dependent component of inositol uptake had a K{sub m} of 50 {mu}M and a V{sub max} of 289 pmol/mg prot/h. Exposure to LiCl, ouabain, or Ca{sup 2+} - free Krebs-Ringer bicarbonate solution inhibited uptake. Metabolic poisoning with dinitrophenol, as well as incubation with phloretin, an inhibitor of carrier-mediated hexose transport, also inhibited uptake. Exposure to norepinephrine decreased inositol uptake, while phorbol myristate acetate was without effect. Isobutylmethylxanthine significantly increased inositol uptake, while the increased uptake due to dibutyryl cyclic AMP and forskolin were not statistically significant. Sodium nitroprusside, and activator of guanylate cyclase, and 8-bromo cyclic GMP, were without effect on uptake, as was methylene blue, an inhibitor of guanylate cyclase. Inositol uptake into the aorta was increased when the endothelium was allowed to remain intact, although this effect was likely due to uptake in both the endothelial and smooth muscle cells.

• OSTI ID: 6694977
• Journal Information: Life Sciences; (USA), Journal Name: Life Sciences; (USA) Vol. 46:23; ISSN 0024-3205; ISSN LIFSA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ADRENAL HORMONES
ALKALI METAL COMPOUNDS
ANIMAL TISSUES
ANIMALS
AORTA
AROMATICS
ARTERIES
AUTONOMIC NERVOUS SYSTEM AGENTS
BIOLOGICAL EFFECTS
BLOOD VESSELS
BODY
CARBOHYDRATES
CARCINOGENS
CARDIOTONICS
CARDIOVASCULAR AGENTS
CARDIOVASCULAR SYSTEM
DRUGS
ENDOTHELIUM
ENZYME INHIBITORS
ESTERS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
INOSITOL
INOSITOLS
MAMMALS
MONOSACCHARIDES
MUSCLES
NEUROREGULATORS
NITRO COMPOUNDS
NITROPHENOL
NORADRENALINE
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHENOLS
PHORBOL ESTERS
RATS
RODENTS
SACCHARIDES
SODIUM COMPOUNDS
SYMPATHOMIMETICS
TISSUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES