Action of tumor initiators and promoters in the Syrian hamster embryo cell transformation assay

Conference · Sun Jun 01 04:00:00 EDT 1986

OSTI ID:6694782

Jones, C A; Huberman, E

The Syrian hamster embryo (SHE) cell transformation assay is unique among the rodent fibroblast transformation systems in that it uses normal, diploid cells. Alteration in the control of growth in carcinogen-treated cultures is used to indicate the onset of neoplastic development. An evaluation of the SHE assay for screening carcinogens is reported. Using coded chemicals, the degree of intra- and interlaboratory reproducibility with the system was evaluated. Overall, there was a good qualitative correlation between the carcinogenicity of the chemicals and their ability to induce morphological cell transformation. Unfortunately, the low level of response and lack of good dose-response relationships with certain chemical are still major constraints to the use of this system in routine testing. Further consideration needs to be given to developing procedures that select for, or amplify, expression of the transformed phenotype. 9 refs., 2 figs., 1 tab.

• Research Organization: Argonne National Lab., IL (USA)
• DOE Contract Number: W-31109-ENG-38
• OSTI ID: 6694782
• Report Number(s): CONF-8606161-2; ON: DE87004968
• Country of Publication: United States
• Language: English

Related Subjects

560300* -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL COMPOUNDS
ANIMAL CELLS
ANIMALS
AROMATICS
BENZOPYRENE
CARCINOGENESIS
CARCINOGENS
CELL TRANSFORMATIONS
CHOLECALCIFEROL
CONDENSED AROMATICS
EMBRYONIC CELLS
ESTERS
FLUORIDES
FLUORINE COMPOUNDS
HALIDES
HALOGEN COMPOUNDS
HAMSTERS
HYDROCARBONS
MAMMALS
ORGANIC COMPOUNDS
PATHOGENESIS
PHORBOL ESTERS
PROMOTERS
PYRENE
RODENTS
SODIUM COMPOUNDS
SODIUM FLUORIDES
SOMATIC CELLS
TUMOR PROMOTERS
VERTEBRATES
VITAMIN D
VITAMINS