Effects of acetylcholine and other agents on /sup 32/P-prelabeled phosphoinositides and phosphatidate in crude synaptosomal preparations

Journal Article · Sun May 01 04:00:00 EDT 1988 · J. Neurosci. Res.; (United States)

DOI:https://doi.org/10.1002/jnr.490200117· OSTI ID:6691737

White, H L

Experimental conditions are described which permit effects of various agents on polyphosphoinositides and phosphatidic acid (PA) to be evaluated simultaneously in crude nerve-ending preparations from rat brain. Acetylcholine (3-100 microM) or carbachol (30-1,000 microM) induced the hydrolysis of prelabeled polyphosphoinositides and, at the same time, stimulated the net label incorporated in phosphatidic acid. All muscarinic effects were blocked by atropine or pirenzepine. Non-muscarinic agonists (glutamate, adenosine, norepinephrine) stimulated polyphosphoinositide hydrolysis in this preparation, but of these only norepinephrine affected phosphatidic acid turnover. A potentiation of acetylcholine-induced phosphoinositide turnover by KCl was observed, as well as an apparent selective inhibition of PIP2 hydrolysis by LiCl. Acetylcholine-stimulated turnover of PA was not necessarily coupled to phosphoinositide hydrolysis.

• Research Organization: Wellcome Research Laboratories, Research Triangle Park, NC (USA)
• OSTI ID: 6691737
• Journal Information: J. Neurosci. Res.; (United States), Journal Name: J. Neurosci. Res.; (United States) Vol. 20:1; ISSN JNRED
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ACETYLCHOLINE
ALKALI METALS
ALKALINE EARTH METALS
ALKALOIDS
AMINES
AMMONIUM COMPOUNDS
ANIMAL CELLS
ANIMALS
ATROPINE
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMICAL REACTION KINETICS
BODY
BRAIN
CALCIUM
CENTRAL NERVOUS SYSTEM
CHLORIDES
CHLORINE COMPOUNDS
DAYS LIVING RADIOISOTOPES
DRUGS
ELEMENTS
ESTERS
HALIDES
HALOGEN COMPOUNDS
ISOTOPE APPLICATIONS
ISOTOPES
KINETICS
LIGHT NUCLEI
LIPIDS
LITHIUM
MAMMALS
METABOLISM
METALS
NERVE CELLS
NERVOUS SYSTEM
NEUROREGULATORS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
OXYGEN COMPOUNDS
PARASYMPATHOLYTICS
PARASYMPATHOMIMETICS
PHOSPHATES
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS COMPOUNDS
PHOSPHORUS ISOTOPES
QUATERNARY COMPOUNDS
RADIOISOTOPES
RATS
REACTION KINETICS
RODENTS
SOMATIC CELLS
TRACER TECHNIQUES
VERTEBRATES