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U.S. Department of Energy
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Microbial screening test for lignite degradation: Quarterly progress report No. 9 for the period January-March 1987

Technical Report ·
OSTI ID:6687580
Anaerobic fermentation of water soluble fraction of modified lignite was attempted. Solubilized lignite formed bioprecipitate after biodegradation. Fermentation of water solubilized lignite in enrichment media produced gases and organic acids. FT-IR spectra of solubilized lignite after biodegradation showed that the concentration of organic oxygen have decreased and that the concentration of -CH/sub 3/ terminal group have increased. Solubilized lignite may serve as sole carbon source by using selective media. Bacteria was suspected of being able to utilize fulvic-like materials from solubilized lignite. Isolation of anaerobic bacteria was achieved by surface culture, and it indicated morphological differences among isolated colonies. Alginate gel entrapment, an immobilization method, was applied to T. versicolor fungal cells. Active fungal growth was observed from the immobilized spheres on sodium-alginate gel. It seems that the immobilized biocatalysts may be used to enhance the production of bioextract from lignite in a reactor system. Hydroxylation of lignite was accomplished through Fenton reaction at pH 7.5. FT-IR analysis showed that lignite treated with Fenton's reagent exhibits weaker aromatic bending and ether linkage than untreated lignite. 13 refs., 8 figs.
Research Organization:
University of Southern California, Los Angeles (USA)
DOE Contract Number:
FG22-84PC70809
OSTI ID:
6687580
Report Number(s):
DOE/PC/70809-T13; ON: DE87007684
Country of Publication:
United States
Language:
English