Microbial screening test for lignite degradation. Quarterly progress report No. 4, October-December 1985

Technical Report · Mon Dec 31 23:00:00 EST 1984

OSTI ID:6328935

Yen, T F

Chemical oxidation of lignite by means of hydrogen peroxide - acetic acid was executed. Methanol fractionation of the oxidized product yielded 76.4% methanol solubles, 9.3% methanol insolubles and 11.2% CO/sub 2/. Biodegradation of the methanol fraction by soil bacteria was found to be positive as demonstrated by gel permeation chromatography (GPC). The shift of the average molecular weight throughout biodegradation was estimated to be from 310 g/mole, to 243 g/mole, zero day and four weeks respectively. Biodegradation of lignitic substrates, benzene - methanol fraction (A - 1), aqueous alkaline fraction (A - 2), clean lignite residue (A - 3), raw lignite, and methanol soluble fraction after oxidation, by means of oil - field soil bacteria and Polyporus versicolor ATCC 12679 was attempted. All previous mentioned lignitic substrates yielded positive results for oil-field soil bacteria, while A - 2 fraction and raw lignite yielded positive results for P. versicolor. Unidentified fungi strains, N 1, L 1, and L 2 were also tested on lignitic substrates. Mild growth was observed in these cases.

• Research Organization: University of Southern California, Los Angeles (USA)
• DOE Contract Number: FG22-84PC70809
• OSTI ID: 6328935
• Report Number(s): DOE/PC/70809-T4; ON: DE86004559
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
010600* -- Coal
Lignite
& Peat-- Properties & Composition
ALCOHOLS
BACTERIA
BIODEGRADATION
BROWN COAL
CARBONACEOUS MATERIALS
CHEMICAL REACTIONS
CHROMATOGRAPHY
COAL
DECOMPOSITION
ENERGY SOURCES
EXTRACTION
FOSSIL FUELS
FRACTIONATION
FUELS
FUNGI
GEL PERMEATION CHROMATOGRAPHY
HYDROXY COMPOUNDS
LIGNITE
MATERIALS
METHANOL
MICROORGANISMS
MOLECULAR WEIGHT
ORGANIC COMPOUNDS
OXIDATION
PLANTS
SEPARATION PROCESSES
SOLVENT EXTRACTION