Preparation and characterization of. beta. -D-glucosidase immobilized in calcium alginate

Journal Article · Thu Dec 31 23:00:00 EST 1981 · Biotechnol. Bioeng. Symp.; (United States)

OSTI ID:6685019

Woodward, J; Krasniak, S R; Smith, R D; Spielberg, F; Zachry, G S

The immobilization of enzymes, organelles, and microbial cells within calcium alginate gel spheres has been reported. Few reports exist, however, on the use of these spheres for enzyme immobilization. The study reported here describes the immobilization of ..beta..-D-glucosidase within calcium alginate gel spheres and addresses the problems of loss of enzyme activity in the spheres via inhibition and leakage.

• Research Organization: Oak Ridge National Lab., TN
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 6685019
• Journal Information: Biotechnol. Bioeng. Symp.; (United States), Journal Name: Biotechnol. Bioeng. Symp.; (United States) Vol. 12; ISSN BIBSB
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400301* -- Organic Chemistry-- Chemical & Physicochemical Properties-- (-1987)
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALGINATES
ALKALINE EARTH METAL COMPOUNDS
CALCIUM COMPOUNDS
CHEMICAL PREPARATION
CHEMICAL PROPERTIES
ENZYME ACTIVITY
ENZYMES
GLUCOSIDASE
GLYCOSYL HYDROLASES
HYDROLASES
IMMOBILIZED ENZYMES
O-GLYCOSYL HYDROLASES
SYNTHESIS