Preparation and characterization of. beta. -D-glucosidase immobilized in calcium alginate

Technical Report · Thu Dec 31 23:00:00 EST 1981

DOI:https://doi.org/10.2172/5599699· OSTI ID:5599699

Krasniak, S. R.; Smith, R. D.

Enzymatic hydrolysis of biomass to produce glucose may become feasible if an inexpensive method to reuse the enzyme can be found. This study investigated one such method whereby ..beta..-D-glucosidase (E.C. 3.2.1.21) was immobilized in calcium alginate gel spheres, which were shown to catalyze the hydrolysis of cellobiose to glucose. There was a loss of 49% of the enzyme from the alginate slurry during gelation. After gelation, in the stable gel spheres, there was a 37% retention of the enzyme activity that was actually immobilized. The reason for the loss in activity was investigated and may be caused by inhibition of the enzyme within the sphere by the calcium cations and the alginate anions also present. Mass transfer effects were minimal in this system and were not responsible for the activity loss.

• Research Organization: Massachusetts Inst. of Tech., Oak Ridge, TN (USA). School of Chemical Engineering Practice
• DOE Contract Number: W-7405-ENG-26
• OSTI ID: 5599699
• Report Number(s): ORNL/MIT-336; ON: DE82006978
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
140504* -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)
550200 -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
ALDEHYDES
ALGINATES
ALKALINE EARTH METAL COMPOUNDS
ANIONS
BIOMASS
CALCIUM COMPOUNDS
CARBOHYDRATES
CATIONS
CELLOBIOSE
CELLULOLYTIC ACTIVITY
CHARGED PARTICLES
CHEMICAL REACTIONS
COLLOIDS
DECOMPOSITION
DISACCHARIDES
DISPERSIONS
ENERGY SOURCES
ENZYMATIC HYDROLYSIS
ENZYME ACTIVITY
ENZYME INHIBITORS
ENZYMES
GELS
GLUCOSE
GLUCOSIDASE
GLYCOSYL HYDROLASES
HEXOSES
HYDROLASES
HYDROLYSIS
IMMOBILIZED ENZYMES
IONS
LYSIS
MONOSACCHARIDES
O-GLYCOSYL HYDROLASES
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
RENEWABLE ENERGY SOURCES
SACCHARIDES
SOLVOLYSIS