Nucleosome structure in chromatin from heated cells

Journal Article · Mon Dec 01 04:00:00 EST 1980 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575488· OSTI ID:6683891

Warters, R L; Roti Roti, J L; Winward, R T

The effect of hyperthermia (40 to 80/sup 0/C) on the nucleosome structure of mammalian chromatin was determined using the enzyme micrococcal nuclease. At equivalent fractional DNA digestion it was found that neither the size of DNA nor the total fraction of cellular DNA associated with nucleosome structure is altered by heat exposure up to 48/sup 0/C for 30 min. It is proposed that this heat-induced reduction in the accessibility to nuclease attack of DNA in chromatin from heated cells is due to the increased protein mass associated with chromatin.

• Research Organization: Univ. of Utah Medical Center, Salt Lake City
• OSTI ID: 6683891
• Journal Information: Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 84:3; ISSN RAREA
• Country of Publication: United States
• Language: English

Related Subjects

560201* -- Thermal Effects-- Cells-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
BACTERIA
BODY TEMPERATURE
CHROMATIN
DNA
ENZYMES
ESTERASES
HYDROLASES
HYPERTHERMIA
MICROCOCCUS
MICROCOCCUS LUTEUS
MICROORGANISMS
NUCLEASES
NUCLEIC ACIDS
NUCLEOPROTEINS
NUCLEOSOMES
ORGANIC COMPOUNDS
PHOSPHODIESTERASES
PROTEINS
SIDE EFFECTS
TEMPERATURE EFFECTS