Enzyme immunoassay for swine trichinellosis using antigens purified by immunoaffinity chromatography

Journal Article · Tue Nov 01 04:00:00 EST 1983 · Am. J. Trop. Med. Hyg.; (United States)

OSTI ID:6671355

Seawright, G L; Despommier, D; Zimmermann, W; Isenstein, R S

Various preparations of crude and a purified preparation of Trichinella spiralis antigens were compared in a rapid, micro-enzyme immunoassay (EIA) for detecting trichinellosis in swine. The crude antigen preparations (XM-300 or S/sub 3/ fraction) were lipid-free, cell-free fractions of muscle larvae, and the purified antigen was prepared by immunoaffinity chromatography of the soluble fraction of stichocyte secretory granules from rat muscle larvae. The antigens were tested against normal and immune swine sera for sensitivity and specificity, and for their ability to detect seroconversions early in the immune response. Tests of sequential sera from experimentally-infected pigs showed that the column antigen produced lower absorbances with pre-infection sera and, from 18 days post-infection, higher absorbances with positive sera. From 21-28 days post-infection, absorbances and S/N ratios with column antigen were nearly twice those with XM-300. Column antigen detected antibodies more often than XM-300 antigen in sera collected prior to the appearance of larvae. Crude antigen did not distinguish all true negatives from weakly positives in a study involving 100 sera from muscle digestion-negative pigs and 75 sera from experimentally infected pigs, whereas the column antigen distinguished all negatives from positives. In a larger scale test of the column antigen, 1130 pigs from Puerto Rico were tested in the micro-EIA test. Puerto Rico has no endogenous trichinellosis, and all 1130 pigs were shown to be muscle digestion negative. These results show that the column antigen out-performs the crude antigens in sensitivity, specificity, and early detection. The column antigen is therefore a major improvement in the EIA for swine trichinellosis.

• Research Organization: Los Alamos National Lab., NM
• OSTI ID: 6671355
• Journal Information: Am. J. Trop. Med. Hyg.; (United States), Journal Name: Am. J. Trop. Med. Hyg.; (United States) Vol. 32:6; ISSN AJTHA
• Country of Publication: United States
• Language: English

Related Subjects

550600* -- Medicine
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIGENS
ASCHELMINTHES
BIOASSAY
CHROMATOGRAPHY
DETECTION
DIAGNOSTIC TECHNIQUES
DOMESTIC ANIMALS
ENZYMES
ETIOLOGY
HELMINTHS
IMMUNE REACTIONS
MAMMALS
NEMATODES
PURIFICATION
SEPARATION PROCESSES
SWINE
TRICHINELLA
VERTEBRATES