Comparative efficacy of antigen and antibody detection tests for human trichinellosis

Journal Article · Wed Feb 01 04:00:00 EST 1989 · J. Parasitol.; (United States)

DOI:https://doi.org/10.2307/3282932· OSTI ID:6173509

Ivanoska, D; Cuperlovic, K; Gamble, H R; Murrell, K D

Sera collected from patients with suspected or confirmed exposure to Trichinella spiralis were tested for circulating parasite antigens and antiparasite antibodies. Using an immunoradiometric assay, excretory--secretory antigens from muscle-stage larvae of T. spiralis were detected in the sera of 47% of 62 patients with clinical trichinellosis and 13% of 39 patients without clinical signs but suspected of exposure to infected meat. In comparison, antibodies were detected using an indirect immunofluorescent test in the circulation of 100% of the 62 patients with clinical trichinellosis and 46% of the 39 patients with suspected exposure. The presence of antibodies specific to excretory-secretory products of T. spiralis muscle larvae was confirmed in the majority of the samples tested by a monoclonal antibody-based competitive inhibition assay. These results indicate that antibody detection is a more sensitive diagnostic method for human trichinellosis, but that antigen detection might be a useful confirmatory test because it is a direct demonstration of parasite products in the circulation.

• Research Organization: Institute of Endocrinology, Immunology and Nutrition, Zemun (Yugoslavia)
• OSTI ID: 6173509
• Journal Information: J. Parasitol.; (United States), Journal Name: J. Parasitol.; (United States) Vol. 75:1; ISSN JOPAA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
ANTIGEN-ANTIBODY REACTIONS
ASCHELMINTHES
COMPARATIVE EVALUATIONS
DIAGNOSIS
DISEASES
FLUORESCENCE
HELMINTHS
IMMUNOASSAY
IMMUNOLOGY
INFECTIOUS DISEASES
ISOTOPE APPLICATIONS
LUMINESCENCE
MAMMALS
MAN
MONOCLONAL ANTIBODIES
MUSCLES
NEMATODES
PARASITIC DISEASES
PATIENTS
PRIMATES
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
SPECIFICITY
TRACER TECHNIQUES
TRICHINELLA
TRICHINOSIS
VERTEBRATES