1-pyrenyldiazomethane as a fluorescent labeling reagent for liquid chromatographic determination of carboxylic acids

Journal Article · Sat Oct 01 00:00:00 EDT 1988 · Anal. Chem.; (United States)

DOI:https://doi.org/10.1021/ac00170a017· OSTI ID:6661607

Nimura, N; Kinoshita, T; Yoshida, T; Uetake, A; Nakai, C

1-Pyrenyldiazomethane (PDAM), a new fluorescent labeling reagent, was developed for liquid chromatographic determination of carboxylic acid. PDAM was more stable than the other previous diazoalkane type reagents. The reagent readily reacted with carboxylic acids to give stable and strongly fluorescent 1-pyrenylmethyl ester derivatives without a catalyst and heating. PDAM derivatives of short- and long-chain fatty acids and prostaglandins were satisfactorily separated on a reversed-phase column and were sensitively detected fluorometrically at 395 nm with excitation at 340 nm. Detection limits of PDAM derivatives of both fatty acids and prostaglandins were 20-30 fmol.

• Research Organization: Taisho Pharmaceutical Co., Ltd., Saitama (Japan)
• OSTI ID: 6661607
• Journal Information: Anal. Chem.; (United States), Journal Name: Anal. Chem.; (United States) Vol. 60:19; ISSN ANCHA
• Country of Publication: United States
• Language: English

Related Subjects

37 INORGANIC, ORGANIC, PHYSICAL, AND ANALYTICAL CHEMISTRY
400102* -- Chemical & Spectral Procedures
ALKANES
CARBOXYLIC ACIDS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
DATA
DIAZO COMPOUNDS
EXPERIMENTAL DATA
FLUORESCENCE
HYDROCARBONS
INFORMATION
LIQUID COLUMN CHROMATOGRAPHY
LUMINESCENCE
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
QUANTITATIVE CHEMICAL ANALYSIS
SEPARATION PROCESSES
TRACE AMOUNTS