DNA sequence of 15 base pairs is sufficient to mediate both glucocorticoid and progesterone induction of gene expression
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
To define the recognition sequence of the glucocorticoid receptor and its relationship with that of the progesterone receptor, oligonucleotides derived from the glucocorticoid response element of the tyrosine aminotransferase gene were tested upstream of a heterologous promoter for their capacity to mediate effects of these two steroids. The authors show that a 15-base-pair sequence with partial symmetry is sufficient to confer glucocorticoid inducibility on the promoter of the herpes simplex virus thymidine kinase gene. The same 15-base-pair sequence mediates induction by progesterone. Point mutations in the recognition sequence affect inducibility by glucocorticoids and progesterone similarly. Together with the strong conservation of the sequence of the DNA-binding domain of the two receptors, these data suggest that both proteins recognize a sequence that is similar, if not the same.
- Research Organization:
- Institute of Cell and Tumor Biology, Heidelberg (Germany, F.R.)
- OSTI ID:
- 6651978
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:22; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
ENZYMES
GENE REGULATION
GLANDS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPES
KETONES
LIGHT NUCLEI
MAMMALS
MAMMARY GLANDS
MAN
MEMBRANE PROTEINS
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PREGNANES
PRIMATES
PROGESTERONE
PROTEINS
RADIOISOTOPES
RECEPTORS
RNA
STEROID HORMONES
STEROIDS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TRANSFERASES
TUMOR CELLS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ADRENAL HORMONES
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CORTICOSTEROIDS
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
ENZYMES
GENE REGULATION
GLANDS
GLUCOCORTICOIDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPES
KETONES
LIGHT NUCLEI
MAMMALS
MAMMARY GLANDS
MAN
MEMBRANE PROTEINS
MESSENGER-RNA
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
OLIGONUCLEOTIDES
ORGANIC COMPOUNDS
ORGANS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PREGNANES
PRIMATES
PROGESTERONE
PROTEINS
RADIOISOTOPES
RECEPTORS
RNA
STEROID HORMONES
STEROIDS
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TRANSFERASES
TUMOR CELLS
VERTEBRATES