Detection of human muscle glycogen by natural abundance /sup 13/C NMR

Journal Article · Tue Mar 01 00:00:00 EST 1988 · Proc. Natl. Acad. Sci. U.S.A.; (United States)

DOI:https://doi.org/10.1073/pnas.85.5.1634· OSTI ID:6651370

Avison, M J; Rothman, D L; Nadel, E; Shulman, R G

Natural abundance /sup 13/C nuclear magnetic resonance spectroscopy was used to detect signals from glycogen in the human gastrocnemius muscle. The reproducibility of the measurement was demonstrated, and the ability to detect dynamic changes was confirmed by measuring a decrease in muscle glycogen levels after exercise and its subsequent repletion. Single frequency gated /sup 1/H decoupling was used to obtain decoupled natural abundance /sup 13/C NMR spectra of the C-1 position of muscle glycogen.

• Research Organization: Yale Univ. School of Medicine, New Haven, CT (USA)
• OSTI ID: 6651370
• Journal Information: Proc. Natl. Acad. Sci. U.S.A.; (United States), Vol. 85:5
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
GLYCOGEN
NMR SPECTRA
MUSCLES
NUCLEAR MAGNETIC RESONANCE
CARBON 13
GLUCOSE
MAN
PROTONS
ALDEHYDES
ANIMALS
BARYONS
CARBOHYDRATES
CARBON ISOTOPES
ELEMENTARY PARTICLES
EVEN-ODD NUCLEI
FERMIONS
HADRONS
HEXOSES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MAMMALS
MONOSACCHARIDES
NUCLEI
NUCLEONS
ORGANIC COMPOUNDS
POLYSACCHARIDES
PRIMATES
RESONANCE
SACCHARIDES
SPECTRA
STABLE ISOTOPES
VERTEBRATES
550501* - Metabolism- Tracer Techniques