Direct observation of glycogen synthesis in human muscle with sup 13 C NMR

Journal Article · Thu Jun 01 00:00:00 EDT 1989 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.86.12.4489· OSTI ID:5432667

Jue, T; Rothman, D L; Shulman, G I; Tavitian, B A; DeFronzo, R A; Shulman, R G ^[1]

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1. Yale Univ., New Haven, CT (USA)

On the basis of previous indirect measurements, skeletal muscle has been implicated as the major site of glucose uptake and it has been suggested that muscle glycogen formation is the dominant pathway. However, direct measurements of the rates of glycogen synthesis have not been possible by previous techniques. The authors have developed {sup 13}C NMR methods to measure directly the rate of human muscle glycogen formation from infused, isotopically labeled (1-{sup 13}C)glucose. They show that under conditions of imposed hyperglycemia and hyperinsulinemia, a majority of the infused glucose was converted to muscle glycogen in a normal man. This directly shows that muscle is the major site of glucose disposal under these conditions, and provides quantitation of the glucose flux to muscle glycogen.

• OSTI ID: 5432667
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 86:12; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
GLYCOGEN
BIOSYNTHESIS
MUSCLES
NUCLEAR MAGNETIC RESONANCE
BIOLOGICAL PATHWAYS
CARBON 13
GLUCOSE
SKELETON
ALDEHYDES
BODY
CARBOHYDRATES
CARBON ISOTOPES
EVEN-ODD NUCLEI
HEXOSES
ISOTOPES
LIGHT NUCLEI
MAGNETIC RESONANCE
MONOSACCHARIDES
NUCLEI
ORGANIC COMPOUNDS
ORGANS
POLYSACCHARIDES
RESONANCE
SACCHARIDES
STABLE ISOTOPES
SYNTHESIS
550601* - Medicine- Unsealed Radionuclides in Diagnostics