Radiolabeling of oligofructans with CO sub 2 in excised wheat leaves

Journal Article · Sat Apr 01 04:00:00 EST 1989 · Plant Physiology, Supplement; (USA)

OSTI ID:6650434

Kanabus, J; Housley, T L; Carpita, N C ^[1]

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1. Purdue Univ., West Lafayette, IN (USA)

The authors studied the flow of carbon ({sup 14}C) from CO{sub 2} through sucrose to oligofructans in wheat (Triticum aestivum L.) leaves that had been induced by excision to accumulate fructan. Leaves were either labeled for 30 min and then chased in ambient air for 2 h or labeled continuously for 2 h. Fructans were chromatographed on BioGel P2 column. During continuous labeling, the specific activity was highest in sucrose and decreased for fructans with DP 3 to 5, and each increased linearly with time for 2 h. During the chase period, sucrose specific activity increased for 60 min and then declined while oligofructans gained in specific activity through the 2 h period. After 30 min of labeling, the specific activity of fructose moiety of sucrose was equal to glucose but, in oligofructans, those of the individual fructose units were not. The results may provide insight into the fructosyl donor-acceptor pool relations in wheat leaves.

• OSTI ID: 6650434
• Journal Information: Plant Physiology, Supplement; (USA), Journal Name: Plant Physiology, Supplement; (USA) Vol. 89:4; ISSN PPYSA; ISSN 0079-2241
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
CARBOHYDRATES
CARBON 14 COMPOUNDS
CEREALS
CHEMICAL REACTIONS
CHROMATOGRAPHY
DISACCHARIDES
GRASS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
LABELLING
LEAVES
LILIOPSIDA
MAGNOLIOPHYTA
OLIGOSACCHARIDES
ORGANIC COMPOUNDS
PHOTOCHEMICAL REACTIONS
PHOTOSYNTHESIS
PLANTS
SACCHARIDES
SACCHAROSE
SEPARATION PROCESSES
SYNTHESIS
TRACER TECHNIQUES
WHEAT