Kestose:sucrose fructosyl transfer - a potential problem in the investigation of fructan and sucrose metabolism

Conference · Tue May 01 04:00:00 EDT 1990 · Plant Physiology, Supplement; (USA)

OSTI ID:5628251

Kanabus, J; Carpita, N C; Housley, T L ^[1]

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1. Purdue Univ., West Lafayette, IN (USA)

Wheat leaves can be induced by excision to produce fructans. Fructose residues of newly made oligofructans in leaves labeled in vivo with {sup 14}CO{sub 2} are not equally labeled. We report here on a fructosyl transferase activity in wheat leaves catalyzing the reaction: G{sup *}-F{sup *} + G-F-F = G{sup *}-F{sup *}-F + G-F. This activity, described previously in J. artichoke was attributed to fructan:fructan fructosyl transferase (FFT). The rate of this reaction in vitro is much higher than that of net kestose synthesis by SST. Hence, appearance of labeled 1-kestose from sucrose may not be an accurate measure of SST, but a curious reshuffling of hexoses between pools of 1-kestose and sucrose.

• OSTI ID: 5628251
• Report Number(s): CONF-9007196--
• Conference Information: Journal Name: Plant Physiology, Supplement; (USA) Journal Volume: 93:1
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
CARBOHYDRATES
CARBON 14 COMPOUNDS
CEREALS
ENZYMES
FRUCTOSE
GRAMINEAE
HEXOSES
ISOTOPE APPLICATIONS
KETONES
KINETICS
LABELLED COMPOUNDS
LILIOPSIDA
MAGNOLIOPHYTA
MONOSACCHARIDES
ORGANIC COMPOUNDS
PLANTS
REACTION KINETICS
SACCHARIDES
TRACER TECHNIQUES
TRANSFERASES
WHEAT