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Model for bile salt micellization and solubilization from studies of a [open quotes]polydisperse[close quotes] array of fluorescent probes and molecular modeling

Journal Article · · Journal of Physical Chemistry; (United States)
DOI:https://doi.org/10.1021/j100102a043· OSTI ID:6649474
;  [1]
  1. Duke Univ., Durham, NC (United States)

The micellar aggregation of the bile salt sodium taurocholate (NaTC) in water, in the concentration range 0-35 mM, was studied by using a [open quotes]polydisperse[close quotes] array of polycyclic aromatic hydrocarbon probes, ranging from pyrene with four aromatic rings to ovalene with 10 aromatic rings. Measurements of fluorescence spectra, peak intensity, vibronic band intensity ratio, and lifetime were used to follow the solubilization of the probes as a function of NaTC concentration. The results strongly support stepwise aggregation behavior since at least two critical regions of NaTC concentration were reported by each of the probes. For all probes, one of these critical regions occurs at 8-12 mM. The other critical concentration regions, referred to as [open quotes]apparent critical micellar concentrations[close quotes] (acmc), increase with increasing probe size and include 4, 15, and 20 mM. 27 refs., 9 figs., 5 tabs.

OSTI ID:
6649474
Journal Information:
Journal of Physical Chemistry; (United States), Journal Name: Journal of Physical Chemistry; (United States) Vol. 98:51; ISSN JPCHAX; ISSN 0022-3654
Country of Publication:
United States
Language:
English