Tris(tetramethylphenanthroline)ruthenium(II): a chiral probe that cleaves A-DNA conformations
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
..lambda..-Tris(3,4,7,8-tetramethyl-1,10-phenanthroline)ruthenium(II) (..lambda..-Ru(TMP)/sub 3//sup 2 +/) was found to be a distinctive molecular tool to examine the local variations in conformation along the strand. The metal complex binds cooperatively to A-form helices of various base sequences under conditions where little or no binding was found to analogous B-form DNAs. Photoactivated DNA cleavage may be coupled to this conformation-specific binding by taking advantage of the photophysical properties of ruthenium(II) complexes. ..lambda..-Ru(TMP)/sub 3//sup 2 +/ cleaves preferentially /sup 3/H-labeled A-form polynucleotides upon irradiation with visible light. The photoinduced DNA strand scission is likely to be mediated by singlet oxygen, which leads to a preferential cleavage of guanine residues. Comparative mapping of cleavage sites on a linear pBR322 fragment for tris(phenanthroline)ruthenium(II), which binds to B-DNA and cleaves also by sensitization of singlet oxygen, and for Ru(TMP)/sub 3//sup 2 +/ shows the selective binding of ..lambda..-Ru(TMP)/sub 3//sup 2 +/ to conformationally distinct sites along the fragment. These sites correspond to 5- to 13-base-pair homopyrimidine stretches.
- Research Organization:
- Columbia Univ., New York, NY (USA)
- OSTI ID:
- 6647712
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 85:5; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
Similar Records
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Thesis/Dissertation
·
Sat Dec 31 23:00:00 EST 1988
·
OSTI ID:6940437
Transition metal complexes as probes for higher-order structure in RNA
Thesis/Dissertation
·
Tue Dec 31 23:00:00 EST 1991
·
OSTI ID:5581076
Proton NMR investigation of tris(phenanthroline)ruthenium(II) enantiomers bound to synthetic deoxyribonucleic acid hexamers
Conference
·
Thu May 01 00:00:00 EDT 1986
· Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)
·
OSTI ID:5022479
Related Subjects
550201 -- Biochemistry-- Tracer Techniques
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CONFORMATIONAL CHANGES
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DNA
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOTOCHEMISTRY
POLYMERIZATION
RADIOISOTOPES
REFRACTORY METAL COMPOUNDS
RUTHENIUM COMPOUNDS
STRAND BREAKS
TRANSITION ELEMENT COMPOUNDS
TRITIUM COMPOUNDS
560300* -- Chemicals Metabolism & Toxicology
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
CHEMICAL REACTIONS
CHEMISTRY
CONFORMATIONAL CHANGES
CROSS-LINKING
DAYS LIVING RADIOISOTOPES
DNA
ISOTOPES
LABELLED COMPOUNDS
LIGHT NUCLEI
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOTOCHEMISTRY
POLYMERIZATION
RADIOISOTOPES
REFRACTORY METAL COMPOUNDS
RUTHENIUM COMPOUNDS
STRAND BREAKS
TRANSITION ELEMENT COMPOUNDS
TRITIUM COMPOUNDS