Proton NMR investigation of tris(phenanthroline)ruthenium(II) enantiomers bound to synthetic deoxyribonucleic acid hexamers

Conference · Thu May 01 04:00:00 EDT 1986 · Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States)

OSTI ID:5022479

Rehmann, J P; Barton, J K

The binding of tris(phenanthroline)ruthenium(II), Ru(phen)/sub 3//sup 2 +/, to DNA is both enantioselective, and DNA sequence and conformation dependent. There is a chiral preference for ..delta..-Ru(phen)/sub 3//sup 2 +/ for the intercalative mode of binding and a surface bound mode shows a weak preference for Lambda-Ru(phen)/sub 3//sup 2 +/. Proton NMR techniques are being used to characterize the solution structure of ..delta..-Ru(phen)/sub 3//sup 2 +/ and Lambda-Ru(phen)/sub 3//sup 2 +/ bound to specific DNA sequences. The Proton NMR resonances of both enantiomers are shifted upfield when bound to the hexamers d(pApT)/sub 3/ and d(pCpG)/sub 3/, and the spectra of the ..delta..- and Lambda-isomers, superimposed in free solution, may be resolved in the presence of DNA. Furthermore, sequence dependent shifts are evident for both enantiomers. Two-dimensional NOESY spectroscopy is being used to elucidate the bound conformation of the DNA-Ru(phen)/sub 3//sup 2 +/ complexes.

• Research Organization: Columbia Univ., New York, NY
• OSTI ID: 5022479
• Report Number(s): CONF-8606151-
• Conference Information: Journal Name: Fed. Proc., Fed. Am. Soc. Exp. Biol.; (United States) Journal Volume: 45:6
• Country of Publication: United States
• Language: English

Related Subjects

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550600 -- Medicine
59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
AROMATICS
AZAARENES
BARYONS
BIOCHEMICAL REACTION KINETICS
CHEMICAL SHIFT
DNA
DNA SEQUENCING
ELEMENTARY PARTICLES
ELEMENTS
ENANTIOMORPHS
FERMIONS
HADRONS
HETEROCYCLIC COMPOUNDS
KINETICS
MAGNETIC RESONANCE
METALS
MOLECULAR STRUCTURE
NMR SPECTRA
NUCLEAR MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENANTHROLINES
PLATINUM METALS
POLYMERS
PROTONS
REACTION KINETICS
RESONANCE
RUTHENIUM
SPECTRA
STRUCTURAL CHEMICAL ANALYSIS
TRANSITION ELEMENTS