Replicon size and rate of fork ovement in early S of higher plant cells (Pisum sativum)

Journal Article · Wed Dec 31 23:00:00 EST 1975 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(76)90275-5· OSTI ID:6646151

Van't Hof, J

Measurements of chromosomal DNA fiber replication of cells of cultured pea root meristems in early S via autoradiography showed a 3-fold increase in rate of fork movement in the first 2 h. The initial rate was 4.5 to 6 ..mu..m h/sup -1/ but forks active after 90 min moved at nearly 18 ..mu..m h/sup -1/. The faster movement was not characteristic of all replicons. Certain fibers consisted of replicons of a smaller mean size (38 to 42 ..mu..m) with slowly moving forks (4.5 to 6 ..mu..m h/sup -1/ fork /sup -1/) and others had replicons almost 50 ..mu..m long with forks that moved more rapidly.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 6646151
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 103; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

550200 -- Biochemistry
550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
CELL CULTURES
CHROMOSOMES
DNA
DNA REPLICATION
FOOD
MERISTEMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PEAS
PLANT TISSUES
ROOTS
VEGETABLES