Pea (Pisum sativum) cells arrested in G2 have nascent DNA with breaks between replicons and replication clusters

Journal Article · Mon Dec 31 23:00:00 EST 1979 · Exp. Cell Res.; (United States)

DOI:https://doi.org/10.1016/0014-4827(80)90346-8· OSTI ID:5729353

Van't Hof, J

DNA fiber autoradiography and alkaline sucrose sedimentation of DNA of cultured pea-root cells (Pisum sativum) arrested in G2 by carbohydrate starvation demonstrated that nascent DNA molecules of replicon (16 to 27 x 10/sup 6/D) and apparent cluster (approx. 330 x 10/sup 6/D) size were not joined. That the arrested cells were in G2 was confirmed by single-cell autoradiography and cytophotometry. In pea there are about 18 replicons per average cluster, 4.2 x 10/sup 3/ clusters, and 7.7 x 10/sup 4/ replicons per genome.

• Research Organization: Brookhaven National Lab., Upton, NY
• OSTI ID: 5729353
• Journal Information: Exp. Cell Res.; (United States), Journal Name: Exp. Cell Res.; (United States) Vol. 129; ISSN ECREA
• Country of Publication: United States
• Language: English

Related Subjects

550201 -- Biochemistry-- Tracer Techniques
550301* -- Cytology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AUTORADIOGRAPHY
BIOCHEMISTRY
CELL CYCLE
CHEMISTRY
DNA
DNA REPLICATION
LABELLED COMPOUNDS
LEGUMINOSAE
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PISUM
PLANT CELLS
PLANTS
STRAND BREAKS
TRITIUM COMPOUNDS