Isolating human DNA repair genes using rodent-cell mutants
Conference
·
OSTI ID:6645179
The DNA repair systems of rodent and human cells appear to be at least as complex genetically as those in lower eukaryotes and bacteria. The use of mutant lines of rodent cells as a means of identifying human repair genes by functional complementation offers a new approach toward studying the role of repair in mutagenesis and carcinogenesis. In each of six cases examined using hybrid cells, specific human chromosomes have been identified that correct CHO cell mutations affecting repair of damage from uv or ionizing radiations. This finding suggests that both the repair genes and proteins may be virtually interchangeable between rodent and human cells. Using cosmid vectors, human repair genes that map to chromosome 19 have cloned as functional sequences: ERCC2 and XRCC1. ERCC1 was found to have homology with the yeast excision repair gene RAD10. Transformants of repair-deficient cell lines carrying the corresponding human gene show efficient correction of repair capacity by all criteria examined. 39 refs., 1 fig., 1 tab.
- Research Organization:
- Lawrence Livermore National Lab., CA (USA); Texas Univ., Smithville (USA). Cancer Center
- DOE Contract Number:
- W-7405-ENG-48
- OSTI ID:
- 6645179
- Report Number(s):
- UCRL-96478; CONF-8703112-1; ON: DE87008735
- Country of Publication:
- United States
- Language:
- English
Similar Records
Human DNA repair and recombination genes
Molecular cloning and biological characterization of a human gene, ERCC2, that corrects the nucleotide excision repair defect in CHO UV5 cells
Correction of xeroderma pigmentosum complementation group D mutant cell phenotypes by chromosome and gene transfer: Involvement of the human ERCC2 DNA repair gene
Conference
·
Thu Sep 01 00:00:00 EDT 1988
·
OSTI ID:7055902
Molecular cloning and biological characterization of a human gene, ERCC2, that corrects the nucleotide excision repair defect in CHO UV5 cells
Journal Article
·
Mon Feb 29 23:00:00 EST 1988
· Mol. Cell. Biol.; (United States)
·
OSTI ID:5131564
Correction of xeroderma pigmentosum complementation group D mutant cell phenotypes by chromosome and gene transfer: Involvement of the human ERCC2 DNA repair gene
Journal Article
·
Tue Dec 31 23:00:00 EST 1991
· Proceedings of the National Academy of Sciences of the United States of America; (United States)
·
OSTI ID:5611589
Related Subjects
550200* -- Biochemistry
550400 -- Genetics
560200 -- Thermal Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CHO CELLS
CLONING
DNA REPAIR
DNA-CLONING
ELECTROMAGNETIC RADIATION
EXCISION REPAIR
GENES
GENETIC MAPPING
MAMMALS
MAN
MAPPING
MUTANTS
PRIMATES
PYRIMIDINE DIMERS
RADIATIONS
RECOVERY
REPAIR
SPECIES DIVERSITY
STRAND BREAKS
ULTRAVIOLET RADIATION
VERTEBRATES
550400 -- Genetics
560200 -- Thermal Effects
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CHO CELLS
CLONING
DNA REPAIR
DNA-CLONING
ELECTROMAGNETIC RADIATION
EXCISION REPAIR
GENES
GENETIC MAPPING
MAMMALS
MAN
MAPPING
MUTANTS
PRIMATES
PYRIMIDINE DIMERS
RADIATIONS
RECOVERY
REPAIR
SPECIES DIVERSITY
STRAND BREAKS
ULTRAVIOLET RADIATION
VERTEBRATES