A V1-vascular vasopressin antagonist suitable for radioiodination and photoaffinity labeling

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · American Journal of Hypertension; (USA)

DOI:https://doi.org/10.1093/ajh/3.6.471· OSTI ID:6644075

Thibonnier, M; Chehade, N; Hinko, A ^[1]

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1. University Hospital of Cleveland, O (USA)

We have previously characterized the V1-vascular arginine vasopressin (AVP) receptors of human platelets. We now report on a radiomonoiodinated and photoreactive V1-vascular AVP antagonist (V1-ag) to be used for the purification of human V1-vascular AVP receptors. The V1-ag, d(CH2)5Tyr(Me)Tyr(NH2)AVP was modified by radiomonoiodination of d(CH2)5-Tyr(Me)Tyr(NH2)AVP with the Iodogen technique, and derivatization of d(CH2)5Tyr(Me)Tyr(125I)(NH2)-AVP with the photoreactive crosslinker, N-hydroxysuccinimidyl-4-azidobenzoate (HSAB) (each step included HPLC purification). In competition experiments, the affinity of these V1-ag for the human platelet AVP receptors remained excellent. Irreversible photoaffinity labeling of the platelet V1-vascular AVP receptor was successfully achieved by UV lamp exposure (365 nm, 20 min). Thus, AzBz-d(CH2)5Tyr(Me)Tyr(125I)(NH2)AVP is a promising tool to use for the purification of human V1-vascular AVP receptors.

• OSTI ID: 6644075
• Journal Information: American Journal of Hypertension; (USA), Journal Name: American Journal of Hypertension; (USA) Vol. 3; ISSN 0895-7061; ISSN AJHYE
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
AFFINITY
ANIMALS
AUTONOMIC NERVOUS SYSTEM AGENTS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BLOOD PLATELETS
BODY FLUIDS
CELL CONSTITUENTS
CELL MEMBRANES
CHEMICAL PREPARATION
CHEMICAL REACTIONS
CHROMATOGRAPHY
DAYS LIVING RADIOISOTOPES
DRUGS
ELECTRON CAPTURE RADIOISOTOPES
FRACTIONATION
HALOGENATION
HORMONES
INTERMEDIATE MASS NUCLEI
IODINATION
IODINE 125
IODINE ISOTOPES
ISOTOPES
LIQUID COLUMN CHROMATOGRAPHY
MAMMALS
MAN
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEI
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
PARASYMPATHOLYTICS
PEPTIDE HORMONES
PITUITARY HORMONES
PRIMATES
PROTEINS
RADIOISOTOPES
RECEPTORS
SEPARATION PROCESSES
SYNTHESIS
VASOPRESSIN
VERTEBRATES