Quantification of diethylstilbestrol residues in meat samples by gas chromatography-isotope dilution mass spectrometry
Journal Article
·
· J. Agric. Food Chem.; (United States)
A method is described for the quantitative determination of residues of the synthetic estrogen diethylstilbestrol (DES) in meat at levels as low as 0.1 ppb. Samples were enzymatically digested with subtilisin and and extracted with diethyl ether. The organic phase was then subjected to a cleanup, consisting of two liquid-liquid partitioning. Residues of DES in the extract were derivatized to bis(heptafluorobutyryl)-DES and assayed by electron-impact mass spectrometry. Quantification was achieved by measuring the intensity ratio of the m/z 660 ion (molecular ion of DES) and the m/z 666 ion (molecular ion of the hexadeuteriated DES analogue). The latter not only served as the internal standard but also monitored the derivatization efficiency and stabilized the cis/trans DES isomers ratio. Analytical recovery from meat, as assayed with a /sup 3/H-labeled DES, was about 90%. The method was used to confirm positive radioimmunoassay results.
- Research Organization:
- Ghent State Univ., Belgium
- OSTI ID:
- 6642666
- Journal Information:
- J. Agric. Food Chem.; (United States), Journal Name: J. Agric. Food Chem.; (United States) Vol. 35:2; ISSN JAFCA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201 -- Biochemistry-- Tracer Techniques
553000* -- Agriculture & Food Technology
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
AROMATICS
CHROMATOGRAPHY
ESTROGENS
FOOD
GAS CHROMATOGRAPHY
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LABELLED COMPOUNDS
MASS SPECTROSCOPY
MEAT
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RESIDUES
SEPARATION PROCESSES
SPECTROSCOPY
STEROID HORMONES
STILBESTROL
TRACER TECHNIQUES
TRITIUM COMPOUNDS
553000* -- Agriculture & Food Technology
59 BASIC BIOLOGICAL SCIENCES
60 APPLIED LIFE SCIENCES
AROMATICS
CHROMATOGRAPHY
ESTROGENS
FOOD
GAS CHROMATOGRAPHY
HORMONES
HYDROXY COMPOUNDS
IMMUNOASSAY
IMMUNOLOGY
ISOTOPE APPLICATIONS
ISOTOPE DILUTION
LABELLED COMPOUNDS
MASS SPECTROSCOPY
MEAT
ORGANIC COMPOUNDS
PHENOLS
POLYPHENOLS
RADIOASSAY
RADIOIMMUNOASSAY
RADIOIMMUNOLOGY
RESIDUES
SEPARATION PROCESSES
SPECTROSCOPY
STEROID HORMONES
STILBESTROL
TRACER TECHNIQUES
TRITIUM COMPOUNDS