Cloning of Bacteroides fragilis plasmid genes affecting metronidazole resistance and ultraviolet survival in Escherichia coli

Journal Article · Thu Mar 01 04:00:00 EST 1990 · Plasmid; (USA)

DOI:https://doi.org/10.1016/0147-619X(90)90035-B· OSTI ID:6640231

Wehnert, G U; Abratt, V R; Goodman, H J; Woods, D R ^[1]

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1. Univ. of Cape Town, Rondebosch (South Africa)

Since reduced metronidazole causes DNA damage, resistance to metronidazole was used as a selection method for the cloning of Bacteroides fragilis genes affecting DNA repair mechanisms in Escherichia coli. Genes from B. fragilis Bf-2 were cloned on a recombinant plasmid pMT100 which made E. coli AB1157 and uvrA, B, and C mutant strains more resistant to metronidazole, but more sensitive to far uv irradiation under aerobic conditions. The loci affecting metronidazole resistance and uv sensitivity were linked and located on a 5-kb DNA fragment which originated from the small 6-kb cryptic plasmid pBFC1 present in B. fragilis Bf-2 cells.

• OSTI ID: 6640231
• Journal Information: Plasmid; (USA), Journal Name: Plasmid; (USA) Vol. 23:2; ISSN PLSMD; ISSN 0147-619X
• Country of Publication: United States
• Language: English

Related Subjects

560130* -- Radiation Effects on Microorganisms
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AEROBIC CONDITIONS
ALCOHOLS
ANTINEOPLASTIC DRUGS
AZOLES
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CONSTITUENTS
CLONING
DNA
DNA REPAIR
DRUGS
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENES
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
IMIDAZOLES
METRONIDAZOLE
MICROORGANISMS
NITRO COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PHENOTYPE
PLASMIDS
RADIATION EFFECTS
RADIATIONS
RADIOSENSITIVITY EFFECTS
RADIOSENSITIZERS
RECOVERY
REPAIR
ULTRAVIOLET RADIATION