Location and cloning of the ultraviolet-sensitizing function from the chromosomally associated IncJ group plasmid, R391

Journal Article · Tue Jul 01 04:00:00 EDT 1986 · Plasmid; (United States)

DOI:https://doi.org/10.1016/0147-619X(86)90076-4· OSTI ID:5002733

Pembroke, J T; Stevens, E; Brandsma, J A; Van de Putte, P

The IncJ plasmid R391, which specifies a uv-sensitizing function, has been shown to be associated with chromosomal DNA. Deletions originating from Tn10 insertion into the kanamycin-resistance determinant of plasmid R391 gave rise to uv-resistant derivatives. This apparent linkage between the kanamycin-resistance determinant and the uv-sensitizing gene(s) was used to clone the uv-sensitizing function from plasmid R391 into pUR222. A recombinant plasmid containing both functions (KanR and Uvs+) was obtained. The uv-sensitizing function was mapped to a 4-kb EcoRI fragment.

• Research Organization: Univ. College, Galway, Ireland
• OSTI ID: 5002733
• Journal Information: Plasmid; (United States), Journal Name: Plasmid; (United States) Vol. 1; ISSN PLSMD
• Country of Publication: United States
• Language: English

Related Subjects

560131* -- Radiation Effects on Microorganisms-- Basic Studies-- (-1987)
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BACTERIA
CELL CONSTITUENTS
CHROMOSOMES
CLONING
DNA
ELECTROMAGNETIC RADIATION
ESCHERICHIA COLI
GENETIC MAPPING
MAPPING
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PLASMIDS
RADIATIONS
RECOMBINANT DNA
SENSITIVITY
ULTRAVIOLET RADIATION