Metabolic processes involved in repair of Escherichia coli cells damaged by exposure to acid mine water

Journal Article · Mon Aug 01 04:00:00 EDT 1988 · Appl. Environ. Microbiol.; (United States)

OSTI ID:6634947

Wortman, A T; Bissonnette, G K

Escherichia coli was stressed by exposure to filter-sterilized acid mine water. Synthetic processes required for repair of sublethally injured survivors were studied by the addition of specific metabolic inhibitors to a resuscitation broth. Inhibitors of protein, RNA, DNA, lipid, and peptidoglycan synthesis as well as uncouplers and inhibitors of electron transport and ATPase activity were used. Acid mine water injury was severe, causing damage to the outer and cytoplasmic membranes. Repair of sublethally injured cells required protein, RNA, and lipid synthesis as well as a proton motive force.

• Research Organization: West Virginia Univ., Morgantown (USA)
• OSTI ID: 6634947
• Journal Information: Appl. Environ. Microbiol.; (United States), Journal Name: Appl. Environ. Microbiol.; (United States) Vol. 54:8; ISSN AEMID
• Country of Publication: United States
• Language: English

Related Subjects

01 COAL, LIGNITE, AND PEAT
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& Peat-- Health & Safety
560300 -- Chemicals Metabolism & Toxicology
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ACID ANHYDRASES
ACID MINE DRAINAGE
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ATP-ASE
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
BIOLOGICAL STRESS
CELL CONSTITUENTS
CELL MEMBRANES
CHLORAMPHENICOL
CYTOPLASM
DNA REPAIR
DNA REPLICATION
DRUGS
ENZYMES
ESCHERICHIA COLI
GENETIC EFFECTS
HYDROLASES
LIPIDS
MEMBRANES
MICROORGANISMS
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHOHYDROLASES
POLLUTION
RECOVERY
REPAIR
RNA
TOXICITY
WATER POLLUTION