T and B lymphocytes of irradiated, tumor-bearing mice treated with an immunostimulator, pyran. [X radiation]

Journal Article · Sun Oct 01 04:00:00 EDT 1978 · Cell. Immunol.; (United States)

DOI:https://doi.org/10.1016/0008-8749(78)90352-0· OSTI ID:6629944

Stylos, W A ^[1]; Chirigos, M A; Lengel, C R; Weiss, J F

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1. National Cancer Inst., Bethesdo, MD

The T and B splenic lymphocyte populations of BALB/c mice were determined in Madison lung 109 carcinoma-bearing animals. Concurrently, some groups of tumored mice were exposed to 500 rads of whole body irradiation and treated with one dose of a nonspecific immunostimulating agent, pyran. By indirect immunofluorescence, it was found that the percentage of splenic T lymphocytes were significantly depressed in the tumored irradiated mice. Mitogenic studies revealed that the PHA-sensitive T lymphocytes were more depressed in the tumored irradiated mice than in the corresponding Con A-sensitive T lymphocytes. Pyran was relatively effective in reconstituting the T cell compartment of these splenic T lymphocytes. The B cell compartment of the splenic lymphocytes of the tumored irradiated mice was found to be extremely radiosensitive. Utilizing a specific anti-F(ab')/sub 2/ serum, no B lymphocytes were detected throughout the duration of testing. Blastogenic studies using LPS as the mitogenic probe revealed that the incorporation of (/sup 3/H)TdR of the tumored irradiated mice was just slightly higher than background values. Pyran proved to be relatively ineffective in reconstituting the splenic B cells of the tumored irradiated mice.

• OSTI ID: 6629944
• Journal Information: Cell. Immunol.; (United States), Journal Name: Cell. Immunol.; (United States) Vol. 40:2; ISSN CLIMB
• Country of Publication: United States
• Language: English

Related Subjects

560152* -- Radiation Effects on Animals-- Animals
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
CARCINOMAS
CONNECTIVE TISSUE CELLS
DISEASES
ELECTROMAGNETIC RADIATION
HETEROCYCLIC COMPOUNDS
IMMUNE REACTIONS
IONIZING RADIATIONS
LEUKOCYTES
LUNGS
LYMPHOCYTES
MAMMALS
MICE
NEOPLASMS
ORGANIC COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANS
PYRANS
RADIATION EFFECTS
RADIATIONS
RESPIRATORY SYSTEM
RODENTS
SOMATIC CELLS
SPLEEN
VERTEBRATES
X RADIATION