Effect of dietary carbohydrates on plasma lipoproteins in Sprague-Dawley and LA/N-corpulent rats

Thesis/Dissertation · Sun Jan 01 04:00:00 EST 1989

OSTI ID:6622836

Ellwood, K C

Male Sprague-Dawley rats were fed diets containing 54% carbohydrate as either sucrose (S), fructose (F) or cooked cornstarch (CS) for 5 weeks. Plasma lipoproteins (LP) were isolated by density gradient ultracentrifugation and separated into very low density P (VLDL), low density LP (LDL) and high density LP (HDL) by gel filtration and affinity chromatography. ApoLP E-rich (R{sub 1} and R{sub 2}) and apoLP E-poor subfractions (NR) of HDL were prepared by heparin-sepharose affinity chromatography. Rats were injected intraperitoneally with {sup 3}H-leucine and sacrificed 2 hours later. Plasma lipoproteins were isolated as described above. The level of {sup 3}H-protein in plasma was greater in Sprague-Dawley rats fed F than those fed S or CS. The amount of {sup 3}H-protein in the chylomicron + VLDL fraction was affected by type of dietary carbohydrate: S > F > CS. Similar studies were conducted with the carbohydrate-sensitive obese and lean LA/N-corpulent rats. Levels of HDL-protein were lower in LA/N-corpulent rats fed S or F than in those fed CS. {sup 3}H-chylomicron + VLDL was higher in rats fed S or F than those fed CS. The concentration of {sup 3}H-protein in plasma and chylomicron + VLDL was greater in obese rats than in lean.

• Research Organization: Maryland Univ., College Park, MD (USA)
• OSTI ID: 6622836
• Country of Publication: United States
• Language: English

Related Subjects

550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ADIPOSE TISSUE
ANIMAL TISSUES
ANIMALS
BIOCHEMICAL REACTION KINETICS
BIOSYNTHESIS
BODY
CARBOHYDRATES
CENTRIFUGATION
CHROMATOGRAPHY
CONNECTIVE TISSUE
DIET
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LIPIDS
LIPOPROTEINS
MAMMALS
ORGANIC COMPOUNDS
PROTEINS
RATS
REACTION KINETICS
RODENTS
SEPARATION PROCESSES
SYNTHESIS
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ULTRACENTRIFUGATION
VERTEBRATES