Skip to main content
U.S. Department of Energy
Office of Scientific and Technical Information

Chemical studies on tobacco smoke. LXIII. Use of radioactive tobacco isolates for studying the formation of smoke components

Journal Article · · J. Agric. Food Chem.; (United States)
DOI:https://doi.org/10.1021/jf60215a023· OSTI ID:6620529
Tobacco plants exposed to /sup 14/CO/sub 2/ during growth served as sources for radioactive phytosterols, neophytadiene, alkaloids, fatty acids and others. Techniques for the isolation and identification of these compounds included solvent distribution, gel-permeation chromatography, thin-layer chromatography in conjunction with radioscanning, and gas chromatography-mass spectrometry. The radioactive isolates were then incorporated into cigarettes, and their distribution patterns in the smoke were determined. A substantial portion of the labeled isolates was combusted to /sup 14/CO/sub 2/, in addition to being transferred intact into the smoke ''tars,'' and degraded to volatile organic compounds. Measurable quantities (less than 1%) were also converted to polynuclear aromatic hydrocarbons. The techniques utilized can serve as models for additional studies of this kind and lead to practical approaches for modifying smoke composition.
Research Organization:
Naylor Dana Inst. for Disease Prevention, Valhalla, NY
OSTI ID:
6620529
Journal Information:
J. Agric. Food Chem.; (United States), Journal Name: J. Agric. Food Chem.; (United States) Vol. 26:1; ISSN JAFCA
Country of Publication:
United States
Language:
English