Human 67-kDa calelectrin contains a duplication of four repeats found in 35-kDa lipocortins
Journal Article
·
· Proc. Natl. Acad. Sci. U.S.A.; (United States)
The 67-kDa calelectrin is the largest number of a family of Ca/sup 2 +/-binding proteins that associate with membranes and phospholipids in a Ca/sup 2 +/-dependent manner. Oligonucleotide probes based on peptide sequences obtained from purified bovine 67-kDa calelectrin were used to screen a human retina cDNA library, and the complete primary structure of human 67-kDa calelectrin was deduced by DNA sequence analysis. The protein consists of eight 68-amino acid repeats separated by linking sequences of variable lengths. It is highly similar to the human lipocortin I and II sequences, each of which contains four such repeats. The amino termini of the three proteins show no sequence similarity; however, in the repeated regions the proteins are 42-45% identical in sequence. Analysis of the 16 repeats from the three proteins provides insights into the structural basis for Ca/sup 2 +/-dependent phospholipid binding. These data place the calelectrins and the lipocortins into the same gene family and suggest that these proteins have similar functions and have evolved from a common ancestor.
- Research Organization:
- Howard Hughes Medical Institute, Dallas, TX (USA)
- OSTI ID:
- 6619112
- Journal Information:
- Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 85:3; ISSN PNASA
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
550201* -- Biochemistry-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
AMINO ACID SEQUENCE
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM COMPOUNDS
CLONING
DAYS LIVING RADIOISOTOPES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAMMALS
MAN
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
PURIFICATION
RADIOISOTOPES
RECOMBINANT DNA
RNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES
59 BASIC BIOLOGICAL SCIENCES
ALKALINE EARTH METAL COMPOUNDS
AMINO ACID SEQUENCE
ANIMALS
ATP
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CALCIUM COMPOUNDS
CLONING
DAYS LIVING RADIOISOTOPES
DNA
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
HYBRIDIZATION
ISOTOPES
LIGHT NUCLEI
LIPIDS
MAMMALS
MAN
MESSENGER-RNA
MOLECULAR STRUCTURE
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDES
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEINS
PURIFICATION
RADIOISOTOPES
RECOMBINANT DNA
RNA
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES