Correlation of gene and protein structure of rat and human lipocortin 1

Journal Article · Tue Sep 17 04:00:00 EDT 1991 · Biochemistry; (United States)

DOI:https://doi.org/10.1021/bi00101a015· OSTI ID:5702823

Kovacic, R T; Tizard, R; Cate, R L; Frey, A Z; Wallner, B P ^[1]

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1. Biogen Inc., Cambridge, MA (United States)

Lipocortins (annexins) are a family of calcium-dependent phospholipid-binding proteins with phospholipase A{sub 2} inhibitory activity. The characteristic primary structure of members of this family consists of a core structure of four or eight repeated domains, which have been implicated in calcium-dependent phospholipid binding. In two lipocortins (1 and 2) a short amino-terminal sequence distinct from the core structure has potential regulatory functions which are dependent on its phosphorylation state. The authors isolated the rat and human lipocortin 1 genes and found that they both consist of 13 exons with a striking conservation of their exon-intron structure and their promoter and amino acid sequences. Both lipocortin I genes are at least 19 kbp in length with exons ranging from 57 to 123 bp interrupted by introns as large as 5 kbp. Each of the four repeat units of lipocortin 1 are encoded by two consecutive exons while individual exons code for the highly conserved putative calcium-binding domains. The promoter sequences in the rat and in human genes are highly conserved and contain nucleotide sequences characterized as enhancer sequences in other genes. The structure of the lipocortin 1 gene lends support to the hypothesis that the lipocortin genes arose by a duplication of a single domain.

• OSTI ID: 5702823
• Journal Information: Biochemistry; (United States), Journal Name: Biochemistry; (United States) Vol. 30:37; ISSN 0006-2960; ISSN BICHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
59 BASIC BIOLOGICAL SCIENCES
AMINO ACID SEQUENCE
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CARBOXYLESTERASES
DAYS LIVING RADIOISOTOPES
DNA SEQUENCING
ENZYME INHIBITORS
ENZYMES
ESTERASES
ESTERS
GENES
HYDROLASES
ISOTOPES
LIGHT NUCLEI
LIPASES
LIPIDS
MAMMALS
MAN
MEMBRANE PROTEINS
MOLECULAR STRUCTURE
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
PHOSPHOLIPIDS
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PRIMATES
PROTEIN STRUCTURE
PROTEINS
RADIOISOTOPES
RATS
RODENTS
STRUCTURAL CHEMICAL ANALYSIS
VERTEBRATES