Targeting of murine radiolabeled monoclonal antibodies in the lymphatics

Journal Article · Wed Apr 15 04:00:00 EDT 1987 · Cancer Res.; (United States)

OSTI ID:6617397

Parker, R J; Keenan, A M; Dower, S K; Steller, M A; Holton, O D; Sieber, S M; Weinstein, J N

The tissue localization of a radiolabeled monoclonal antibody directed against a mouse Class I major histocompatibility antigen has been determined in mice following i.v. and s.c. administration. When labeled antibody was given s.c., radioactivity rapidly accumulated in regional lymph nodes draining the injection site, allowing visualization of the nodes by gamma camera imaging within minutes of injection. At 2 h after s.c. injection, radioactivity in regional nodes was present largely as intact antibody, but considerable degradation of antibody present in nodes was noted by 12 h after injection. Since little of the radioactivity reached the blood stream, visualization of regional nodes was possible for long periods after dosing. In contrast, antibody given i.v. showed no significant accumulation in lymph nodes at any time after dosing.

• Research Organization: National Cancer Institute, Bethesda, MD
• OSTI ID: 6617397
• Journal Information: Cancer Res.; (United States), Journal Name: Cancer Res.; (United States) Vol. 8; ISSN CNREA
• Country of Publication: United States
• Language: English

Related Subjects

550601* -- Medicine-- Unsealed Radionuclides in Diagnostics
62 RADIOLOGY AND NUCLEAR MEDICINE
ANIMALS
ANTIBODIES
BIOLOGICAL LOCALIZATION
COUNTING TECHNIQUES
DIAGNOSTIC USES
DISTRIBUTION
INJECTION
INTAKE
LYMPH NODES
LYMPHATIC SYSTEM
MAMMALS
MICE
MOLECULAR WEIGHT
MONOCLONAL ANTIBODIES
RADIOISOTOPE SCANNING
RODENTS
SUBCUTANEOUS INJECTION
TISSUE DISTRIBUTION
USES
VERTEBRATES