Investigations on renal organic and inorganic solutes, in vivo
A basic question in renal physiology is how do the cells of the renal medulla survive the high concentrations of sodium chloride and urea which occur with antidiuresis. The problem is two-fold: (1) urea, being highly permeable to cell membranes, should enter the cell and adversely affect protein function; and (2) inorganic ions, being in much higher concentration extracellularly than intracellularly should dehydrate the cell. If these organic solutes exist in response to high concentrations of sodium chloride and urea, then their content should vary with diuretic state. Two protocols were developed to test the validity of this hypothesis. The first protocol used {sup 31}P-NMR in vivo to monitor GPC content before, during, and after acute diuresis in an exteriorized rabbit kidney model. Changes in sodium distribution and tissue structure were monitored dynamically with {sup 23}Na- and {sup 1}H-NMR imaging, respectively. The second protocol used HPLC to quantitate each of the four organic solutes in renal inner medullary homogenates. Here, the effect of diuretic state and acute diuresis on organic solute content was assessed.
- Research Organization:
- Duke Univ., Durham, NC (USA)
- OSTI ID:
- 6617089
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
62 RADIOLOGY AND NUCLEAR MEDICINE
CELL MEMBRANES
PERMEABILITY
KIDNEYS
PHYSIOLOGY
DYNAMIC FUNCTION STUDIES
LIQUID COLUMN CHROMATOGRAPHY
NMR IMAGING
PHOSPHORUS 31
PROTONS
RABBITS
SODIUM 23
SODIUM CHLORIDES
UREA
ALKALI METAL COMPOUNDS
ALKALI METAL ISOTOPES
AMIDES
ANIMALS
BARYONS
BODY
CARBONIC ACID DERIVATIVES
CELL CONSTITUENTS
CHLORIDES
CHLORINE COMPOUNDS
CHROMATOGRAPHY
DIAGNOSTIC TECHNIQUES
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HALIDES
HALOGEN COMPOUNDS
ISOTOPES
LIGHT NUCLEI
MAMMALS
MEMBRANES
NUCLEI
NUCLEONS
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PHOSPHORUS ISOTOPES
SEPARATION PROCESSES
SODIUM COMPOUNDS
SODIUM ISOTOPES
STABLE ISOTOPES
VERTEBRATES
551001* - Physiological Systems- Tracer Techniques
550600 - Medicine