sup 1 H NMR study of renal trimethylamine responses to dehydration and acute volume loading in man

Journal Article · Mon Jul 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.14.6053· OSTI ID:6100222

Avison, M J; Rothman, D L; Nixon, T W; Long, W S; Siegel, N J ^[1]

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1. Yale Univ. School of Medicine, New Haven, CT (United States)

The authors have used volume-localized {sup 1}H NMR spectroscopy to detect and measure changes in medullary trimethylamines (TMAs) in the human kidney in vivo. Localized water-suppressed {sup 1}H spectra were collected from a volume of interest located within the renal medulla by using a stimulated echo-based localization scheme. The principal resonances in the medullary {sup 1}H spectrum were residual water, lipid, and TMAs. The TMA line width was 7-15 Hz before filtering, and the signal-to-noise ratio was 40:1. In four normal volunteers, 15 hr of dehydration led to a significant increase in urine ismolality and decrease in body weight and an increase in medullary TMAs. A subsequent water load caused a transient water diuresis, a return to euvolemic body weight, and a significant reduction in medullary TMAs within 4 hr. These results suggest that TMAs may play an osmoregulatory role in the medulla of the normal human kidney.

• OSTI ID: 6100222
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:14; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
KIDNEYS
DEHYDRATION
NUCLEAR MAGNETIC RESONANCE
METHYLAMINE
PHYSIOLOGY
PROTONS
URINE
AMINES
BARYONS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY
BODY FLUIDS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
MAGNETIC RESONANCE
MATERIALS
NUCLEONS
ORGANIC COMPOUNDS
ORGANS
RESONANCE
WASTES
550201* - Biochemistry- Tracer Techniques