Identification and characterization of a mutation affecting the division arrest signaling of the pheromone response pathway in Saccharomyces cerevisiae

Journal Article · Thu Feb 01 00:00:00 EST 1990 · Genetics; (USA)

OSTI ID:6614427

Fujimura, Hiroaki ^[1]

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1. Developmental and Reproductive Biology Center, Yamagata (Japan) Hoechst Japan Ltd., Kawagoe (Japan)

Mating pheromones, a- and {alpha}-factors, arrest the division of cells of opposite mating types, {alpha} and a cells, respectively. The author has isolated a sterile mutant of Saccharomyces cerevisiae using EMS that is defective in division arrest in response to {alpha}-factor but not defective in morphological changes and agglutinin induction. The mutation was designated dac2 for division arrest control by mating pheromones. The dac2 mutation was closely linked to gal1 and was different from the previously identified cell type nonspecific sterile mutations (ste4, ste5, ste7, ste11, ste12, ste18, and dac1). Although dac2 cells had no phenotype in the absence of pheromones, they showed morphological alterations and divided continuously in the presence of pheromones. As a result, dac2 cells had a mating defect. The dac2 mutation could suppress the lethality caused by the disruption of the GPA1 gene. These results suggest that the DAC2 product may control the signal for G-protein-mediated cell-cycle arrest and indicate that the synchronization of haploid yeast cell cycles by mating pheromones is essential for cell fusion during conjugation.

• OSTI ID: 6614427
• Journal Information: Genetics; (USA), Vol. 124:2; ISSN 0016-6731
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
EMS
GENETIC EFFECTS
GENE MUTATIONS
GENETIC MAPPING
SACCHAROMYCES CEREVISIAE
CELL DIVISION
AGGLUTININS
CELL CYCLE
HAPLOIDY
MORPHOLOGICAL CHANGES
PHEROMONE
PROTEINS
ANTIBODIES
BIOLOGICAL EFFECTS
ESTERS
EUMYCOTA
FUNGI
MAPPING
MICROORGANISMS
MUTAGENS
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
PLANTS
PLOIDY
SACCHAROMYCES
SECRETION
SULFONIC ACID ESTERS
YEASTS
560300* - Chemicals Metabolism & Toxicology