Citric acid production using immobilized conidia of Aspergillus niger TMB 2022
Conidia of Aspergillus niger TMB 2022 were immobilized in calcium alginate for the production of citric acid. A 1-ml condidia suspension containing ca. 2.32 x 10/sup 8/ conidia were entrapped into sodium alginate solution in order to prepare 3% Ca-alginate (w/v) gel bead. Immobilized conidia were inoculated into productive medium containing 14% sucrose, 0.25% (NH/sub 4/)/sub 2/CO/sub 3/, 0.25% KH/sub 2/PO/sub 4/, and 0.025% MgSO/sub 4/.7H/sub 2/O with addition of 0.06 mg/l CuSO/sub 4/.5H/sub 2/O, 0.25 mg/l ZnCl/sub 2/, 1.3 mg/l FeCl/sub 3/.6H/sub 2/O, pH 3.8, and incubated at 35 degrees C for 13 days by surface culture to produce 61.53 g/l anhydrous citric acid. Under the same conditions with a batchwise culture, it was found that immobilized conidia could maintain a longer period for citric acid production (31 days): over 70 g/l anhydrous citric acid from runs No. 2-4, with the maximum yield for anhydrous citric acid reaching 77.02 g/l for run No. 2. In contrast, free conidia maintained a shorter acid-producing phase, circa 17 days; the maximum yield for anhydrous citric acid was 71.07 g/l for run No. 2 but dropped quickly as the run number increased. 14 references.
- Research Organization:
- National Taiwan Univ., Taipei, Republic of China
- OSTI ID:
- 6614167
- Journal Information:
- Biotechnol. Bioeng.; (United States), Vol. 29:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
09 BIOMASS FUELS
CITRIC ACID
BIOSYNTHESIS
SPORES
IMMOBILIZED CELLS
ALGINATES
ASPERGILLUS
BATCH CULTURE
BIOCONVERSION
COMPARATIVE EVALUATIONS
CULTURE MEDIA
PRODUCTIVITY
CARBOXYLIC ACIDS
FUNGI
HYDROXY ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SYNTHESIS
550700* - Microbiology
140504 - Solar Energy Conversion- Biomass Production & Conversion- (-1989)