A genetic map of chromosome 20q12-q13. 1: Multiple highly polymorphic microsatellite and RFLP markers linked to the maturity-onset diabetes of the Young (MODY) locus

Journal Article · Fri Jan 01 04:00:00 EST 1993 · American Journal of Human Genetics; (United States)

OSTI ID:6613541

Rothschild, C B; Akots, G; Hayworth, R; Pettenati, M J; Rao, P N; Wood, P ^[1]; Stolz, F M; Hansmann, I ^[2]; Serino, K; Keith, T P ^[3]; Fajans, S S ^[4]

---

1. Wake Forest Univ., Winston-Salem, NC (United States)
2. Universitaet Goettingen (Germany)
3. Collaborative Research Inc., Waltham, MA (United States)
4. Univ. of Michigan Medical Center, Ann Arbor (United States)

Multiple highly polymorphic markers have been used to construct a genetic map of the q12-q13.1 region of chromosome 20 and to map the location of the maturity-onset diabetes of the young (MODY) locus. The genetic map encompasses 23 cM and includes 11 loci with PIC values >.50, seven of which have PICs >.70. New dinucleotide repeat polymorphisms associated with the D20S17, PPGB, and ADA loci have been identified and mapped. The dinucleotide repeat polymorphisms have increased the PIC of the ADA locus to .89 and, with an additional RFLP at the D20S17 locus, the PIC of the D20S17 locus to .88. The order of the D20S17 and ADA loci determined genetically (cen-ADA-D20S17-qter) was confirmed by multicolor fluorescence in situ hybridization. The previously unmapped PPGB marker is closely linked to D20S17, with a two-point lod score of 50.53 at [cflx [theta]] = .005. These markers and dinucleotide repeat markers associated with the D20S43, D20S46, D20S55, D20S75, and PLC1 loci and RFLPs at the D20S16, D20S17, D20S22, and D20S33 have been used to map the MODY locus on chromosome 20 to a 13-cM (sex averaged) interval encompassing ADA, D20S17, PPGB, D20S16, and D20S75 on the long arm of chromosome 20 and to create a genetic framework for additional genetic and physical mapping studies of the region. With these multiple highly polymorphic loci, any MODY family of appropriate size can be tested for the chromosome 20 linkage. 34 refs., 3 figs., 3 tabs.

• OSTI ID: 6613541
• Journal Information: American Journal of Human Genetics; (United States), Journal Name: American Journal of Human Genetics; (United States) Vol. 52:1; ISSN AJHGAG; ISSN 0002-9297
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
CHROMOSOMES
DIABETES MELLITUS
DISEASES
DNA HYBRIDIZATION
ENDOCRINE DISEASES
FLUORESCENCE
GENETIC MAPPING
HUMAN CHROMOSOMES
HYBRIDIZATION
LUMINESCENCE
MAPPING
MAPS
METABOLIC DISEASES
RFLPS