Definition and mapping of STSs at STR and RFLP loci in Xp11-Xq22

Journal Article · Wed Dec 01 04:00:00 EST 1993 · Genomics; (United States)

DOI:https://doi.org/10.1016/S0888-7543(05)80381-7· OSTI ID:7076990

Barker, D F; Fain, P R ^[1]

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1. Univ. of Utah Medical School, Salt Lake City, UT (United States)

New primer pair sequences specific for 25 loci in the xp11-q22.1 region are described. Eighteen of the pairs span segments containing significant CA dinucleotide repeats, with 9 of these revealing polymorphisms of greater than 50% heterozygosity. Four of the CA-containing segments occur in probes previously reported to detect RFLPs, while the remaining 14 are from newly isolated clones. STSs were also developed for 7 other RFLP-only loci. All of these 25 STSs plus 11 other published STR markers have been fine-mapped with respect to chromosomal breakpoints, defining 15 subintervals in Xp11-Xq22. This map of 36 STSs, nearly all of which are associated with markers that are genetically mapped and/or highly polymorphic, will significantly aid efforts to construct a complete physical map of this region and to correlate it with the high-density genetic map. 35 refs., 1 fig., 1 ref.

• DOE Contract Number: FG02-88ER60689
• OSTI ID: 7076990
• Journal Information: Genomics; (United States), Journal Name: Genomics; (United States) Vol. 18:3; ISSN GNMCEP; ISSN 0888-7543
• Country of Publication: United States
• Language: English

Related Subjects

550400* -- Genetics
59 BASIC BIOLOGICAL SCIENCES
BIOLOGICAL MARKERS
CHROMOSOMES
GENETIC MAPPING
HETEROCHROMOSOMES
HUMAN CHROMOSOMES
HUMAN X CHROMOSOME
MAPPING
X CHROMOSOME