Isolation of putative cDNA clones of the alternative oxidase of Sauromatum guttatum
- Michigan State Univ., East Lansing (USA)
Antigenically related protein of 35, 36, and 37 kDa are involved in the alternative respiratory pathway in the appendix of S. guttatum. Polyclonal antibodies specific to these proteins immunoprecipitated a 42 kDa putative precursor from total in vitro translated products. Monoclonal and polyclonal antibodies were used to identify clones from a S. gluttatum cDNA expression library. These clones were capable of hybrid-selecting polyA+ RNA transcripts which, when translated in vitro, produced a 42 kDa protein that can be immunoprecipitated by the polyclonal antibodies. These data suggest that the cDNA clones encode one or more of the alternative oxidase proteins of S. guttatum. The clones will be used to investigate the developmental expression of the alternative oxidase proteins in S. guttatum.
- OSTI ID:
- 6611970
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 89:4; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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