Use of archived tissues for studies of plutonium-induced lung tumors
Previous lifespan studies in rats exposed to plutonium-239 aerosols indicated that lung tumor incidence might be increased at radiation doses to the lung comparable to doses received by humans from a maximum permissible occupational lung deposition of 0.6 kBq STZPu. A total of 3,192 young adults, female, SPF, Wistar rats were used in the initial lifespan study: 2,134 were exposed to STZPuO2 at initial lung burdens (ILB) ranging from 0.009 to 6.7 kBq, and 1,058 were sham-exposed controls. Histopathological analyses have been completed on 1707 of the 3,192 rats, including 54 sham-exposed control sand 1153 exposed animals. Cell kinetics, autoradiographic and morphometric techniques are being used to evaluate the spatial-temporal dose-distribution patterns and the cellular events leadings up to lung tumor formation in 140 serially sacrificed female, Wistar rats given a single exposure to STZPuO2 (ILB, 3.9 kBq). Protooncogene activation, growth factors and growth factor receptors, DNA cell content (by cell flow cytometry and microspectrophotometry) and cell proliferation (by TH-TdR nuclear labeling) are being examined in archival paraffin-block sections. 27 refs., 2 figs.
- Research Organization:
- Pacific Northwest Lab., Richland, WA (USA)
- DOE Contract Number:
- AC06-76RL01830
- OSTI ID:
- 6608753
- Report Number(s):
- PNL-SA-16470; CONF-8810209-9; ON: DE89005617
- Resource Relation:
- Conference: 27. Hanford life sciences symposium on health and the environment, Richland, WA, USA, 18 Oct 1988; Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
NEOPLASMS
RADIOINDUCTION
PLUTONIUM 239
BIOLOGICAL EFFECTS
AEROSOLS
EVALUATION
INHALATION
LIFE SPAN
MORTALITY
RATS
SPATIAL DISTRIBUTION
STORAGE LIFE
TEMPORAL DOSE DISTRIBUTIONS
ACTINIDE ISOTOPES
ACTINIDE NUCLEI
ALPHA DECAY RADIOISOTOPES
ANIMALS
COLLOIDS
DISEASES
DISPERSIONS
DISTRIBUTION
EVEN-ODD NUCLEI
HEAVY NUCLEI
INTAKE
ISOTOPES
MAMMALS
NUCLEI
PLUTONIUM ISOTOPES
RADIATION DOSE DISTRIBUTIONS
RADIOISOTOPES
RODENTS
SOLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560151* - Radiation Effects on Animals- Man