Interaction of phytic acid and zinc affecting copper bioavailability in rats
The objectives of this investigation were to develop a protocol to measure Cu bioavailability using four different indices of Cu status, and to evaluate the effect of phytic acid on Cu bioavailability using these indices in the rat. Ninety-six Sprague-Dawley male weanling rats were fed a Cu-deficient diet for four weeks. The Cu-depleted rats were divided into twelve groups and fed test diets containing 1, 2, 3, and 10 ug Cu and 0, 0.4 and 0.8% phytic acid at each Cu level. After 3 days of Cu repletion, liver copper (LCu), liver superoxide dismutase (LSOD) activity, serum copper (SCu), and serum ceruloplasmin (CP) were measured. There was a significant decrease in SCu and LCu in rats fed 30 ug Zn/g or 230 ug Zn/g compared to animals fed 12 ug Zn/g. Phytic acid increased the level of Cu in the sera and livers of rats fed the diet with 30 ug Zn/g, but those of rats fed 12 or 230 ug Zn/g did not. Dietary phytic acid appeared to reduce the synthesis of two intestinal proteins: a high molecular weight protein and metallothionein. The incorporation of /sup 35/S-cysteine into both proteins increased with increasing levels of dietary Zn. The results of these studied indicated: (1) LCu and SCu are reliable indices of Cu for absorption and utilization in the rat, and (2) phytic acid increases Cu bioavailability by binding dietary Zn and reduces the level of Zn-induced mucosal proteins which allows Cu to be more efficiently absorbed.
- Research Organization:
- Missouri Univ., Columbia (USA)
- OSTI ID:
- 6608552
- Resource Relation:
- Other Information: Thesis (Ph. D.)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
59 BASIC BIOLOGICAL SCIENCES
CERULOPLASMIN
BLOOD CHEMISTRY
COPPER COMPOUNDS
BIOLOGICAL AVAILABILITY
PHYTIC ACID
BIOLOGICAL EFFECTS
ZINC COMPOUNDS
CYSTEINE
LIVER
NUTRITIONAL DEFICIENCY
RATS
SULFUR 35
SUPEROXIDE DISMUTASE
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
COMPLEXES
COPPER COMPLEXES
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ENZYMES
ESTERS
EVEN-ODD NUCLEI
GLANDS
GLOBULINS
GLOBULINS-ALPHA
ISOTOPES
LIGHT NUCLEI
MAMMALS
METALLOPROTEINS
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANIC SULFUR COMPOUNDS
ORGANS
OXIDOREDUCTASES
PHOSPHORIC ACID ESTERS
PROTEINS
RADIOISOTOPES
RODENTS
SULFUR ISOTOPES
THIOLS
TRANSITION ELEMENT COMPLEXES
TRANSITION ELEMENT COMPOUNDS
VERTEBRATES
560300* - Chemicals Metabolism & Toxicology
550201 - Biochemistry- Tracer Techniques