Vascular filtration function in galactose-fed versus diabetic rats: The role of polyol pathway activity

Journal Article · Sun Jul 01 00:00:00 EDT 1990 · Metabolism, Clinical and Experimental; (USA)

DOI:https://doi.org/10.1016/0026-0495(90)90102-I· OSTI ID:6606622

Pugliese, G; Tilton, R G; Speedy, A; Chang, K; Province, M A; Kilo, C; Williamson, J R ^[1]

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1. Washington Univ. School of Medicine, St Louis, MO (USA)

These studies were undertaken to assess the effects of increased galactose (v increased glucose) metabolism via the polyol pathway on vascular filtration function in the kidneys, eyes, nerves, and aorta. Quantitative radiolabeled tracer techniques were used to assess glomerular filtration rate (GFR) and regional tissue vascular clearance of plasma 131I-bovine serum albumin (BSA) in five groups of male Sprague-Dawley rats: nondiabetic controls, streptozotocin-diabetic rats, nondiabetic rats fed a 50% galactose diet, diabetic rats treated with sorbinil (an aldose reductase inhibitor), and galactose-fed rats treated with sorbinil. Sorbinil was added to the diet to provide a daily dose of approximately .2 mmol/kg body weight. After 2 months of diabetes or galactose ingestion, albumin clearance was increased twofold to fourfold in the eye (anterior uvea, choroid, and retina), sciatic nerve, aorta, and kidney; GFR was increased approximately twofold and urinary excretion of endogenous albumin and IgG were increased approximately 10-fold. Sorbinil treatment markedly reduced or completely prevented all of these changes in galactose-fed, as well as in diabetic rats. These observations support the hypothesis that increased metabolism of glucose via the sorbitol pathway is of central importance in mediating virtually all of the early changes in vascular filtration function associated with diabetes in the kidney, as well as in the eyes, nerves, and aorta. On the other hand, renal hypertrophy in diabetic rats and polyuria, hyperphagia, and impaired weight gain in galactose-fed and in diabetic rats were unaffected by sorbinil and therefore are unlikely to be mediated by increased polyol metabolism.

• OSTI ID: 6606622
• Journal Information: Metabolism, Clinical and Experimental; (USA), Vol. 39:7; ISSN 0026-0495
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
BLOOD VESSELS
BIOLOGICAL FUNCTIONS
DIABETES MELLITUS
PATHOGENESIS
GALACTOSE
METABOLISM
ALBUMINS
AORTA
BIOLOGICAL PATHWAYS
EDTA
ENZYME INHIBITORS
EYES
IODINE 131
KIDNEYS
NERVES
RATS
TRACER TECHNIQUES
ALDEHYDES
AMINO ACIDS
ANIMALS
ARTERIES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
BODY AREAS
CARBOHYDRATES
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CHELATING AGENTS
DAYS LIVING RADIOISOTOPES
DISEASES
ENDOCRINE DISEASES
FACE
FUNCTIONS
HEAD
HEXOSES
INTERMEDIATE MASS NUCLEI
IODINE ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
METABOLIC DISEASES
MONOSACCHARIDES
NERVOUS SYSTEM
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIOISOTOPES
RODENTS
SACCHARIDES
SENSE ORGANS
VERTEBRATES
550901* - Pathology- Tracer Techniques