Use of Ti plasmid DNA probes for determining tumorigenicity of agrobacterium strains

Journal Article · Fri Jun 01 00:00:00 EDT 1990 · Applied and Environmental Microbiology; (USA)

OSTI ID:6606545

Burr, T J; Norelli, J L; Katz, B H; Bishop, A L ^[1]

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1. Cornell Univ., Geneva, NY (USA)

Probes consisting of T-DNA genes from the Ti plasmid of Agrobacterium tumefaciens were used for determining tumorigenicity of strains. Two {sup 32}P-labeled probes hybridized with 28 of 28 tumorigenic strains of the pathogen but not with 20 of 22 nontumorigenic strains. One probe, pTHE17, consists of all but the far left portion of the T-DNA of strain C58. Probe SmaI7 consists of SmaI fragment 7 of pTiC58, including onc genes 1, 4, and 6a and most of 2. Another probe, pAL4044, consisting of the vir region of strain Ach-5, hybridized with several nontumorigenic as well as tumorigenic strains. Colony hybridizations were done with 28 tumorigenic and 22 nontumorigenic Agrobacterium strains. About 10{sup 6} CFU of the different tumorigenic strains were detectable with this method. Southern analyses confirmed the presence or absence of Ti plasmids in strains for which tumorigenicity was questioned. Colony hybridization with the T-DNA probes provides a rapid and sensitive means for determining the tumorigenic nature of Agrobacterium strains.

• OSTI ID: 6606545
• Journal Information: Applied and Environmental Microbiology; (USA), Vol. 56:6; ISSN 0099-2240
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
DNA
BIOASSAY
PATHOGENS
CARCINOGENESIS
BACTERIA
GENES
HYBRIDIZATION
PHOSPHORUS 32
PLASMIDS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
CELL CONSTITUENTS
DAYS LIVING RADIOISOTOPES
ISOTOPES
LIGHT NUCLEI
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
PATHOGENESIS
PHOSPHORUS ISOTOPES
RADIOISOTOPES
550901* - Pathology- Tracer Techniques