Early burn wound excision and skin grafting postburn trauma restores in vivo neutrophil delivery to inflammatory lesions

Journal Article · Thu Dec 01 04:00:00 EST 1988 · Arch. Surg. (Chicago); (United States)

DOI:https://doi.org/10.1001/archsurg.1988.01400360047007· OSTI ID:6602994

Tchervenkov, J I; Epstein, M D; Silberstein, E B; Alexander, J W

This study assessed the effect of early vs delayed postburn wound excision and skin grafting on the in vivo neutrophil delivery to a delayed-type hypersensitivity (DTH) reaction and a bacterial skin lesion (BSL). Male Lewis rats were presensitized to keyhole-limpet hemocyanin. Group 1 comprised sham controls. Groups 2 through 4 were given a 30% 3 degrees scald burn, but the burn wounds were excised, and skin was grafted on days 1, 3, and 7, respectively, after the burn. Group 5 comprised burn controls. Twelve days after burn trauma, all rats were injected at different intervals (during a 24-hour period) with a trio of intradermal injections of keyhole-limpet hemocyanin, Staphylococcus aureus 502A, and saline at different sites. In vivo neutrophil delivery to these dermal lesions was determined by injecting indium in 111 oxyquinoline-labeled neutrophils isolated from similarly treated groups of rats. Neutrophil delivery to DTH and BSL lesions was restored to normal by excision and skin grafting of the burn wound one day after burn trauma. Waiting three days after burn trauma to excise and skin graft the wound partially, but not completely, restored the in vivo neutrophil delivery to DTH and BSL lesions. Waiting one week to excise and skin graft a burn wound resulted in no improvement in neutrophil delivery to DTH and BSL dermal lesions. It was concluded that burn wound excision and skin grafting immediately after burn trauma restored in vivo neutrophil delivery to a BSL and DTH dermal lesion. This may, in part, explain the beneficial effect of early aggressive burn wound debridement in patients with burn injuries.

• Research Organization: Univ. of Cincinnati, OH (USA)
• OSTI ID: 6602994
• Journal Information: Arch. Surg. (Chicago); (United States), Journal Name: Arch. Surg. (Chicago); (United States) Vol. 123:12; ISSN ARSUA
• Country of Publication: United States
• Language: English

Related Subjects

550901* -- Pathology-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BIOLOGICAL RECOVERY
BLOOD
BLOOD CELLS
BODY
BODY FLUIDS
BURNS
CELL PROLIFERATION
DAYS LIVING RADIOISOTOPES
ELECTRON CAPTURE RADIOISOTOPES
GRAFTS
HEALING
INDIUM 111
INDIUM ISOTOPES
INFLAMMATION
INJURIES
INTERMEDIATE MASS NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NEUTROPHILS
NUCLEI
ODD-EVEN NUCLEI
ORGANS
PATHOLOGICAL CHANGES
RADIOISOTOPES
RATS
RECOVERY
RODENTS
SKIN
SYMPTOMS
TRACER TECHNIQUES
TRANSPLANTS
VERTEBRATES
WOUNDS