Mitochondrial transcription complex from Saccharomyces cerevisiae

Journal Article · Tue Feb 10 04:00:00 EST 1981 · J. Biol. Chem.; (United States)

OSTI ID:6601510

Levens, D; Morimoto, R; Rabinowitz, M

A DNA protein complex has been isolated from the mitochondria of Saccharomyces cerevisiae. The complex transcribes RNA complementary to mtDNA in a nonrandom manner. The RNA polymerase activity contained in the transcription complex is not dependent on the addition of exogenous template. The activity is rendered template-dependent by autolysis and can be further purified by heparin-Sepharose 4B chromatography. The activity is inhibited by heparin, Mn/sup 2 +/, and increasing ionic strength. The activity requires Mg/sup 2 +/ and ribonucleotides. The preferred template for the template dependent activity is poly(d(AT)). The majority of the RNA synthesized by the transcription complex from endogenous DNA is complementary to the DNA strands directing the synthesis of the large and small ribosomal RNA. In yeast the 21 S and 14 S rRNA genes are widely separated, therefore the transcription of these two regions but not of the intervening regions by the transcription complex suggests the existence of at least two transcriptional promoters on the yeast mitochondrial genome.

• Research Organization: Univ. of Chicago, IL
• OSTI ID: 6601510
• Journal Information: J. Biol. Chem.; (United States), Journal Name: J. Biol. Chem.; (United States) Vol. 256:3; ISSN JBCHA
• Country of Publication: United States
• Language: English

Related Subjects

550200* -- Biochemistry
550700 -- Microbiology
59 BASIC BIOLOGICAL SCIENCES
BIOCHEMICAL REACTION KINETICS
BIOCHEMISTRY
CELL CONSTITUENTS
CHEMISTRY
DNA
DNA REPLICATION
ENZYME ACTIVITY
ENZYMES
FUNGI
KINETICS
MESSENGER-RNA
MICROORGANISMS
MITOCHONDRIA
MOLECULAR STRUCTURE
NUCLEIC ACID REPLICATION
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ORGANIC COMPOUNDS
ORGANOIDS
PHOSPHORUS-GROUP TRANSFERASES
PLANTS
POLYMERASES
REACTION KINETICS
RNA
SACCHAROMYCES
SACCHAROMYCES CEREVISIAE
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TRANSFERASES
YEASTS