Isolation of xylose reductase gene of Pichia stipitis and its expression in Saccharomyces cerevisiae
Conference
·
OSTI ID:431591
- Osaka Univ. (Japan); and others
A NADPH/NADH-dependent xylose reductase gene was isolated from the xylose-assimilating yeast, Pichia stipitis. DNA sequence analysis showed that the gene consists of 951 bp. The gene introduced in Saccharomyces cerevisiae was transcribed to mRNA, and a considerable amount of enzyme activity was observed constitutively, whereas transcription and translation in P steps were inducible. S. cerevisiae carrying the xylose reductase gene could not, however, grow on xylose medium, and could not produce ethanol from xylose. Since xylose uptake and accumulation of xylitol by S. cerevisiae were observed, the conversion of xylitol to xylulose seemed to be limited.
- Research Organization:
- Oak Ridge National Lab., TN (United States); Badger Engineers, Inc., Tampa, FL (United States); Solar Energy Research Inst., Golden, CO (United States)
- OSTI ID:
- 431591
- Report Number(s):
- CONF-900512--
- Country of Publication:
- United States
- Language:
- English
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