Fluorescent DNA probes for flow cytometry: considerations and prospects

Journal Article · Mon Jan 01 04:00:00 EST 1979 · J. Histochem. Cytochem.; (United States)

DOI:https://doi.org/10.1177/27.12.391999· OSTI ID:6600774

Crissman, H A; Orlicky, D J; Kissane, R J

Techniques employing base specific deoxyribonucleic acid (DNA)-binding fluorochromes and flow cytometry (FCM) are potentially useful for obtaining information of the compositional features of chromatin or chromosomes of mammalian cells. Fluorescent compounds which form complexes preferentially at the A-T rich regions or the G-C rich regions in DNA are available and compatible with current FCM technology as are other compounds which show little or no base specificity and bind by intercalation in the double stranded regions of helical DNA. Since extrinsic fluorescent probes provide only a measure of available binding sites or regions unobstructed by chromatin-associated or chromosomal-associated proteins, interpretations of fluorescence measurements need to be substantiated by adequate control measures.

• Research Organization: Los Alamos Scientific Lab., NM
• OSTI ID: 6600774
• Journal Information: J. Histochem. Cytochem.; (United States), Journal Name: J. Histochem. Cytochem.; (United States) Vol. 27:12; ISSN JHCYA
• Country of Publication: United States
• Language: English

Related Subjects

550300* -- Cytology
59 BASIC BIOLOGICAL SCIENCES
CELL FLOW SYSTEMS
CHROMATIN
CHROMOSOMES
CLATHRATES
DNA
DYES
EMISSION SPECTROSCOPY
EVALUATION
FLUORESCENCE SPECTROSCOPY
NUCLEIC ACIDS
ORGANIC COMPOUNDS
SPECTROSCOPY