Specific suppression of anti-DNA production in vitro
Journal Article
·
· J. Clin. Immunol.; (United States)
To investigate the regulation of anti-DNA antibody production, we generated anti-DNA-specific suppressor cells by exposing normal human T cells and a small percentage of adherent cells to high concentrations of DNA. These cells suppressed the production of anti-DNA by both autologous peripheral blood mononuclear cells (PBMC) and allogeneic PBMC derived from systemic lupus erythematosus (SLE) patients. Anti-DNA production was suppressed significantly more than anti-RNA, antitetanus, or total immunoglobulin production. Specific suppression was enhanced by increasing the numbers of DNA-primed CD8+ cells and was obliterated by irradiation of the DNA-primed cells. In contrast to T cells from normal individuals, T cells obtained from two intensively studied SLE patients were unable to generate specific suppressor cells for anti-DNA production in both autologous and allogeneic test systems. Despite this defect, these patients were still capable of generating specific suppressor cells for antibody production directed against an exogenous antigen, tetanus toxoid.
- Research Organization:
- Harbor-UCLA Medical Center, Torrance (USA)
- OSTI ID:
- 6599588
- Journal Information:
- J. Clin. Immunol.; (United States), Journal Name: J. Clin. Immunol.; (United States) Vol. 8:5; ISSN JCIMD
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
560120* -- Radiation Effects on Biochemicals
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IMMUNOSUPPRESSION
IN VITRO
IRRADIATION
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MAMMALS
MAN
MATERIALS
MONOCYTES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
PHAGOCYTES
PRIMATES
RADIATION EFFECTS
RHEUMATIC DISEASES
RNA
SKELETAL DISEASES
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
Cells
& Tissue Culture
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ANIMAL CELLS
ANIMALS
ANTIBODIES
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL RADIATION EFFECTS
BIOSYNTHESIS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
DISEASES
DNA
GENETIC EFFECTS
GENETIC RADIATION EFFECTS
IMMUNOSUPPRESSION
IN VITRO
IRRADIATION
LEUKOCYTES
LYMPHOCYTES
MACROPHAGES
MAMMALS
MAN
MATERIALS
MONOCYTES
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PATIENTS
PHAGOCYTES
PRIMATES
RADIATION EFFECTS
RHEUMATIC DISEASES
RNA
SKELETAL DISEASES
SOMATIC CELLS
SYNTHESIS
VERTEBRATES